- ￥99 - 999
- Sigma
- 进口
- 50531-10G
- 2025年11月24日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
常温
- 保质期：
见标签
- 库存：
现货
- 供应商：
联硕
- CAS号：
53956-04-0
- 规格：
10G
旋光性
[α]20/D +48±4°, c = 1% in ethanol: water (1:1)
甜味
50 × sucrose
杂质
≤7% water
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文献和实验setting) and collect the mononuclear cell (MNC) (buffy coat) layer. Resuspend and wash MNCs x 3 with Dulbecco's Phosphate Buffered Saline (DPBS) with 10% Fetal Bovine Serum (FBS) x 10 min, 515 g, before plating into two 12-well plates in full EPC growth
straightforward assayprotocols using immunoblotting. 2. Materials 2.1. Cell Culture and Lysis 1. MiaPaCa2 and Panc04.03 pancreatic cancer cells are maintainedin DMEM and RPMI-1640 (Gibco/BRL, Bethesda, MD),respectively, supplemented with 10% fetal bovine
DNA extraction from Mutation Detection Enhancement (MDE) Gel Stained with Silver Nitrate
by centrifugation at 12000-¡Á g for 5 minutes. The pellet is completely air dried and dissolved in 10-20µl H2O overnight at 37¡ãC. All reagents were purchased from sigma (Corning®; Sigma Chemical). The yield of DNA from a single band was usually too week
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