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上海帝博思生物科技有限公司成立于上海,主要目的是为了服务生物科学,医学,微生学物等各行业,公司提供各类产品包括:TPP,THOMSO 等耗材,INTEGRA,Satorious,KUHER,compodock,miele,GONOTEC等仪器,各类产品上海帝博思生物科技有限公司均有大量现货。经过不断的磨砺,上海帝博思生物科技有限公司开发出自有品牌:帝博思(TIPS)移液器,摇床,市场占有率迅猛增长。各类常用产品公司均有大量现货。
名称:5000 µL, 1 Bag of 100 Tips, Individually Wrapped, Sterile, BULK
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文献和实验ES Cell Culture and Manipulation
(the DNA will probably come right out of solution so freezing isn't needed). Pellet the DNA, wash with 70% EtOH and dry the pellet. Resuspend the DNA in 100µl sterile H20 or TE (you should be working in the hood here). Check 0.5 µl DNA on a gel, and make
ES Cell Culture and Manipulation
isn't needed). Pellet the DNA, wash with 70% EtOH and dry the pellet. Resuspend the DNA in 100µl sterile H20 or TE (you should be working in the hood here). Check 0.5 µl DNA on a gel, and make sure that the concentration is know. Preparation
with a micropipette tip and then vortex mixing vigorously. 8. Pipette 10 and 100 µl samples onto plates of appropriate SC selection medium, incubate at 30°C for 3-4 days and isolate transformants. The 10 µl samples should be pipetted into 100 µl puddles of sterile
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