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- 2026年03月24日
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文献和实验9. Cover the openings of the deep well plates with Qiagen Airpore Tape Sheets and place the plastic lid over the sheet. Place the plates in a 37°C shaker incubator at 200 RPM for twenty-four hours. 10. Add 50 µl of 45% (w/v) sterile glycerol
ES Cell Culture and Manipulation
tips (preferably barrier, or plugged tips), a sterile 96well round-bottom plate, plenty of ES cell media, and an inverted microscope in a hood. Aspirate the REF media from the 24-wells and add 2ml of ES cell media to each well. Add 40 µl trypsin-EDTA
ES Cell Culture and Manipulation
isn't needed). Pellet the DNA, wash with 70% EtOH and dry the pellet. Resuspend the DNA in 100µl sterile H20 or TE (you should be working in the hood here). Check 0.5 µl DNA on a gel, and make sure that the concentration is know. Preparation
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