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文献和实验Extraction of DNA using DNAzol® Reagent
the culture plate and gently pipet the lysate into an assay tube. Cell Pellets or Suspensions: Add 1 ml of DNAzol® Reagent to 1-3 × 107 cells, either in pellet or in suspension (volume 100 µl), pellet the cells and wash them with 0.9% NaCl. Pellet
Endothelial Cell Systems - Instructions For Use
the harvested cells at 220 x g for 5 minutes to pellet the cells. a. Aspirate most of the supernatant, except for 100-200 µl. b.Flick the cryovial with your finger to loosen the pellet. Dilute the cell pellet in 4-5 ml of growth medium and note the total
dishes (e.g., Corning® CellSTACK®). 6. CO 2 incubator and biosafety cabinet. 7. Filter bottle, 0.5–1 L, 0.2-μm pore size (Corning or equivalent). 2.2. Preparation of Wnt3A CM for Fractionation 1. 20% (v/v) Triton X-100. 2. 1 M Tris-HCl, pH
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