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- 2026年01月26日
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文献和实验Gene Knockout with Conventional Mutagens
overnight. 10. Count the healthy F1 L1s in a 10 ul aliquot. There will be a lot of sick or dead ones (their parents were mutagenized) -- don't count these. Determining numbers of F1s 11. Put 1250 F1 L1s on each of 110 6 cm AENG plates
, as it will reduce the viability of the cultures. 2. Prepare sets of standard 96 well round (U) bottom plates by labeling all plates and placing 100 µl of LB broth containing 100ug/ml carbenicillin in each well. These plates will be used as working copies
Procedures for In Vitro Production of Bovine Embryos
) 5 ml sterile pipets (13-676-10H) 10 ml sterile pipets (13-676-10J) 25 ml sterile pipets (13-676-10K) Pipet tips 200 mL pipet tips (960 (10 racks of 96) sterile tips) (02-681-142)200 m L pipet tips (bulk) (02-681-140
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