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文献和实验PROTOCOL TO EXTRACT DNA FROM PA
fixed paraffin samples into eppendorf tubes. 2.Add 1 ml xylene, mix, incubate at 55℃ for 15mins. Release pressure, spin down for 2minutes in eppendorf ultramicrofuge, pipet off & discard supernatent. 3.Repeat Step 2. 4.Add 100
PROTOCOL TO EXTRACT DNA FROM PA
of DNA. Glycogen makes a nice visible pellet] and 2-2.5X volume with 100% ethanol. Let sit at RT for 2 hours or overnight at -20℃.(better O.N.). 13.Spin for 20minutes at 4℃, decant ethanol (rinse pellet with etoh if it doesn't move), blot dry, air dry
The ribonuclease protection assay (RPA)
ammonium acetate and 650 µl ice cold 100% ethanol. Mix by inverting the tubes and incubate for 30 minutes at -70°C. Spin in the microfuge for 5 minutes at 4°C. 19. Carefully remove the supernatant and add 100 µl ice cold 90% ethanol. Spin
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