Anti-HLA-DMB antibody

Anti-HLA-DMB antibody

Cat. No.     JC264186
Package    25 μl/100 μl/200 μl
Storage    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Product overview
Description                   Anti-HLA-DMB rabbit polyclonal antibody
Applications        ELISA, WB, IHC 
Immunogen               Synthetic peptide of human HLA-DMB
Reactivity                 Human
Content                     0.6 mg/ml
Host species               Rabbit
Ig class                  Immunogen-specific rabbit IgG
Purification                 Antigen affinity purification

Target information
Symbol                 HLA-DMB
Full name                 major histocompatibility complex, class II, DM beta
Synonyms    RING7; D6S221E
Swissprot    P28068

Target Background
HLA-DMB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta (DMB) chain, both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP (class II-associated invariant chain peptide) molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail.

Applications
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human tonsil
Recommended dilution: 25-100    
            
The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using ml264186(HLA-DMB Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200)    
Western blotting
Predicted band size:29 kDa
Positive control:Raji cell lysate 
Recommended dilution: 200-1000