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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海联迈生物工程有限公司
- 库存:
大量
- 靶点:
详见说明书
- 级别:
1
- 目录编号:
LM-10236R-FITC
- 克隆性:
多克隆
- 抗原来源:
Rabbit
- 保质期:
1年
- 抗体英文名:
Anti-Sialoadhesin/FITC
- 抗体名:
Anti-Sialoadhesin/FITC
- 标记物:
FITC标记
- 宿主:
Human, Mouse,
- 适应物种:
Human, Mouse,
- 免疫原:
详见说明书
- 亚型:
IGg
- 形态:
粉末、液体、冻干粉
- 应用范围:
ICC=1:50-200 IF=1:50-200
- 浓度:
1mg/ml
- 保存条件:
-20 °C
- 规格:
100ul
| 英文名称 | Anti-Sialoadhesin/FITC |
| 中文名称 | FITC标记的唾液酸结合性免疫球蛋白样凝集素1抗体 |
| 别 名 | CD169; CD 169; CD-169; CD169 antigen; dJ1009E24.1; DKFZp667F058; FLJ00051; FLJ00055; FLJ00073; FLJ00411; FLJ32150; Sialic acid binding Ig like lectin 1; Sialic acid binding immunoglobulin like lectin 1; Siglec 1; Siglec1; SN; sialoadhesin; SN_HUMAN. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 免疫学 微生物学 b-淋巴细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, |
| 产品应用 | ICC=1:50-200 IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 186kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Sialoadhesin |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a lectin-like adhesion molecule that binds glycoconjugate ligands on cell surfaces in a sialic acid-dependent manner. It is a type I transmembrane protein expressed only by a subpopulation of macrophages and is involved in mediating cell-cell interactions. Alternative splicing produces a transcript variant encoding an isoform that is soluble rather than membrane-bound; however, the full-length nature of this variant has not been determined. [provided by RefSeq, Jul 2008]. Function: Acts as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Preferentially binds to alpha-2,3-linked sialic acid. Binds to SPN/CD43 on T-cells. May play a role in hemopoiesis. Subcellular Location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted. Tissue Specificity: Expressed by macrophages in various tissues. High levels are found in spleen, lymph node, perivascular macrophages in brain and lower levels in bone marrow, liver Kupffer cells and lamina propria of colon and lung. Also expressed by inflammatory macrophages in rheumatoid arthritis. Similarity: Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. Contains 16 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 Ig-like V-type (immunoglobulin-like) domain. Database links: Entrez Gene: 6614 Human Omim: 600751 Human SwissProt: Q9BZZ2 Human Unigene: 31869 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
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文献和实验1 前言 唾液酸主要存在于高等动物的细胞表面,是糖蛋白、糖脂等复合糖的组成成分,担任重要的生物学功能1)。另外,众所周知唾液酸糖链是流感病毒的受体,它像病原性微生物一样被人体识别。 传统方法检测α 2-3结合型唾液酸糖链的非还原糖链末端通常使用MAM或者MAH凝集素。这里的凝集素末端具有Sia α 2-3 Gal β 1- 4GlcNAc 结构,能与N结合型糖链的3链、4链紧密结合2)。但凝集素对糖脂反应性低,对单糖、二糖的结合性低,且难以广泛的检测与不同苷元
无关,是独立遗传的,是另外的血型系统。而且M、N与P也不是一个系统。控制不同血型系统的血型基因在不同的染色体上,即使在一个染色体上,两个系统的基因位点也相距甚远,不是连锁关系,因此是独立遗传的。 有些血型抗体是不完全抗体,与相应的抗原细胞结合后看不出凝集现象,血清中有抗体但不容易发现。1945年抗人球蛋白试验应用到血型检查中来,这种试验就可检查不完全抗体,从此,许多血型抗原陆续被人发现。每当发现一个新抗原后就要确定这一抗原与已经发现的血型是什么关系,这样在人的红细胞上便确定了若干
精神症状有关的抗神经元抗体等。免疫病理学检查包括皮肤狼疮带试验和肾脏免疫荧光检查,皮肤狼疮带试验可见表真皮交界处有免疫球蛋白(1gG、IgM、IgA等)和补体(C3c、Clq等)沉积,肾脏免疫荧光多呈现多种免疫球蛋白和补体成分沉积,被称为“满堂亮”。 免疫荧光法检查SI-E患者血清中的抗核抗体 不同患者的抗核抗体的特异性不同,所显示的核型也不同。 用患者血清为一抗,FITC标记的芋抗人IsG作二抗,在荧光显微镜下观察。 SLE的病因尚不明确,但普遍
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