Anti-DAGLA antibody

Anti-DAGLA antibody

Cat. No.     JC263668
Package    25 μl/100 μl/200 μl
Storage    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Product overview
Description                   Anti-DAGLA rabbit polyclonal antibody
Applications        ELISA, IHC 
Immunogen               Synthetic peptide of human DAGLA
Reactivity                 Human, Mouse, Rat
Content                     0.8 mg/ml
Host species               Rabbit
Ig class                  Immunogen-specific rabbit IgG
Purification                 Antigen affinity purification

Target information
Symbol                 DAGLA
Full name                 diacylglycerol lipase alpha
Synonyms    NSDDR; C11orf11; DAGLALPHA; DAGL(ALPHA)
Swissprot    Q9Y4D2

Target Background
Members of the AB hydrolase superfamily have diverse catalytic functions and play a crucial role in the metabolism of lipids. DAGLα (diacylglycerol lipase alpha), also known as NSDDR or C11orf11, is a 1,042 amino acid multi-pass membrane protein that belongs to the AB hydrolase superfamily. Highly expressed in brain and pancreas, DAGLα uses calcium as a cofactor to catalyze the hydrolysis of diacylglycerol (DAG) to 2-arachidonoyl-glycerol (2-AG), a reaction that is required for axonal growth and for retrograde synaptic signaling at mature synapses. DAGLα functions as at optimal pH of 7 and its activity is inhibited by p-hydroxy-mercuri-benzoate and HgCl2. The gene encoding DAGLα maps to human chromosome 11, which houses over 1,400 genes and comprises nearly 4% of the human genome.

Applications
Immunohistochemistry
Predicted cell location: Cytoplasm or Nucleus
Positive control: Human liver cancer
Recommended dilution: 25-100    Predicted cell location: Cytoplasm or Nucleus
Positive control: Human esophagus cancer
Recommended dilution: 25-100
            
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using ml263668(DAGLA Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200)    The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using ml263668(DAGLA Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200)

ELISA
Recommended dilution: 5000-10000