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- 2025年12月15日
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- 保存条件：
常温
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见标签
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现货
- 供应商：
联硕
- CAS号：
521984-48-5
- 规格：
5mg
生化/生理作用
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文献和实验Establishment of Stable Transfectant of CHO Lec Cells
expression vectors with strong promoter such as immediate early CMV (pcDNA3.1 etc.) or human EF-1a (pEF1/V5-His etc.). pBJ-5/GS vector also gives you high expression but requires additional cloning strategy. Materials Medium Sigma provides
Establishment of Stable Transfectant of CHO Lec Cells
must be done over a couple of medium changes (i.e., change to 5% FCS once and to 2% twice followed by completely switching to serum-free medium etc.). In the case of Sigma's media being discontinued (which happened before), you can prepare basic medium (J/J
will not grow in Schneider's medium from Sigma] 50 ml Fetal Bovine Serum (JRH #12103-78P) - Heat Inactivated (aliquoted in -20) 5 ml 1:100 Penicillin-Streptomycin (Gibco #15070-063) (aliquoted in -20) Sterile filter (0.2 µ) Store at 4o C
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