Anti-CARD16 antibody

Anti-CARD16 antibody

Cat. No.     JC263494
Package    25 μl/100 μl/200 μl
Storage    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Product overview
Description                   Anti-CARD16 rabbit polyclonal antibody
Applications        ELISA, WB, IHC 
Immunogen               Synthetic peptide of human CARD16
Reactivity                 Human
Content                     0.6 mg/ml
Host species               Rabbit
Ig class                  Immunogen-specific rabbit IgG
Purification                 Antigen affinity purification

Target information
Symbol                 CARD16
Full name                 caspase recruitment domain family member 16
Synonyms    COP; COP1; PSEUDO-ICE
Swissprot    Q5EG05

Target Background
Caspase inhibitor. Acts as a regulator of procaspase-1/CASP1 activation implicated in the regulation of the proteolytic maturation of pro-interleukin-1 beta (IL1B) and its release during inflammation. Inhibits the release of IL1B in response to LPS in monocytes. Also induces NF-kappa-B activation during the pro-inflammatory cytokine response. Also able to inhibit CASP1-mediated neuronal cell death, TNF-alpha, hypoxia-, UV-, and staurosporine-mediated cell death but not ER stress-mediated cell death. Acts by preventing activation of caspases CASP1 and CASP4, possibly by preventing the interaction between CASP1 and RIPK2.

Applications
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human liver cancer
Recommended dilution: 25-100    Predicted cell location: Cytoplasm
Positive control: Human ovarian cancer
Recommended dilution: 25-100
            
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using ml263494(CARD16 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: ×200)    The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using ml263494(CARD16 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: ×200)
Western blotting
Predicted band size:23 kDa
Positive control:HEPG2 cell lysate 
Recommended dilution: 200-1000
Gel: 12%SDS-PAGE
Lysate: 40 μg
Lane: HEPG2 cell lysate
Primary antibody: ml263494(CARD16 Antibody) at dilution 1/400
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 60 seconds