Anti-MUC1(NT) antibody

Anti-MUC1(NT) antibody

Cat. No.     JC262654
Package    25 μl/100 μl/200 μl
Storage    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Product overview
Description                   Anti-MUC1(NT) rabbit polyclonal antibody
Applications        ELISA, IHC 
Immunogen               Synthetic peptide of human MUC1(NT)
Reactivity                 Human
Content                     0.7 mg/ml
Host species               Rabbit
Ig class                  Immunogen-specific rabbit IgG
Purification                 Antigen affinity purification

Target information
Symbol                 MUC1(NT)
Full name                 mucin 1, cell surface associated
Synonyms    EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC
Swissprot    P15941

Target Background
This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.

Applications
Immunohistochemistry
Predicted cell location: Cytoplasm or Nucleus
Positive control: Human liver cancer
Recommended dilution: 25-100    Predicted cell location: Cytoplasm or Nucleus
Positive control: Human thyroid cancer
Recommended dilution: 25-100
            
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using ml262654(MUC1(NT) Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200)    The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using ml262654(MUC1(NT) Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200)

ELISA
Recommended dilution: 2000-5000