- $840
- LSBio
- 0.312 - 20 ng/ml
- LS-F39043
- 美国
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 样本：
血清/血浆/组织匀浆液
- 库存：
1000
- 适应物种：
Human
- 应用：
科研使用
- 检测方法：
ELISA
- 检测范围：
0.312 - 20 ng/ml
- 供应商：
康朗生物
- 规格：
96T
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Product Description
LS-F39043 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human Gasdermin D / GSDMD in samples of Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of Gasdermin D / GSDMD as low as 0.116 nanograms per millilter.Specifications
TypeSandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
Gasdermin D / GSDMD
Reactivity
Human
Manual
Intended Sample Types
Tissue Homogenates
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
0.312 - 20 ng/ml
Sensitivity
0.116 ng/ml
Assay Time
4.5 h
Precision
Intra-Assay: CV<10% Inter-Assay: CV<12%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- User Manual
- 96-well Microplate
- Detection Reagents
- Wash Buffer
- Substrate
- Stop Solution
- Adhesive Plate Sealers
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
About Gasdermin D / GSDMD
P57764 NM_024736 NP_079012.3
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Sandwich ELISA Platform Overview
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9. GlutaMAX™-I Supplement 10. Basic FGF, Recombinant Human 11. β-Mercaptoethanol, 1000X 12. Penicillin-Streptomycin, Liquid 13. Attachment Factor 14. TrypLE™ Select Cell Dissociation Reagent or 0.05% Trypsin/EDTA
264.7 细胞进行 EST12-His pull-down 实验，WB 分析；B：共聚焦显微镜分析分别转染 pEGFP-C1-EST12 和 pAsRED2-C1-RACK1 的 RAW264.7 细胞；C：WB 和 qPCR 分析 2μＭ EST12 刺激的 RAW264.7 细胞；D、E：LDH、ELISA 分析 2μＭ EST12 处理 WT 和 RACK1−/−腹腔巨噬细胞上清；F：WB 检测细胞裂解液中 GSDMD 和 caspase-1；G：2μＭ EST12 处理和 PI 染色 WT、RACK
有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂，增加了操作步骤，在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法：1， TORCH及传染病试剂盒（间接法），见2.2.32， TORCH-IgM捕获法特色：包被抗体，标记抗原原理：3， 细胞因子试剂盒采用的方法路线（ABC-ELISA）原理产品特色：采用ABC法，灵敏度更高，特异性更强。生物素抗体和酶联物是浓缩的，使用前需用相应的缓冲液稀释。酶联物可以通用
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