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- 详细信息
- 文献和实验
- 技术资料
- 样本:
血清/血浆/组织匀浆液
- 库存:
1000
- 适应物种:
Rat
- 应用:
科研使用
- 检测方法:
ELISA
- 检测范围:
6.25 - 1600 ng/ml
- 供应商:
康朗生物
- 规格:
96T
Rat IgM ELISA Kit (Competitive EIA)
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Product Description
LS-F54213 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Rat IgM in samples of Plasma and Serum. It is based upon a Competitive EIA assay principle and can be used to detect levels of IgM as low as 6.25 nanograms per millilter.Specifications
TypeCompetitive EIA (enzyme immunoassay) kit
Target
IgM
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of rat IgM. No significant cross-reactivity or interference between rat IgM and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between rat IgM and all the analogues, therefore, cross reaction may still exist.
Manual
Intended Sample Types
Plasma, Serum
Sample Size
50 - 100 µl
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
6.25 - 1600 ng/ml
Sensitivity
6.25 ng/ml
Assay Time
2.5 h
Precision
Intra-Assay: CV<8% / Inter-Assay: CV<10%
Storage
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- User Manual
- 96-well Microplate
- Detection Reagents
- Wash Buffer
- Substrate
- Stop Solution
- Adhesive Plate Sealers
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
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Competition ELISA Platform Overview
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 小鼠IgM 定量 EIA 试剂盒使用说明 原理 本实验采用双抗体夹心 ELISA 法。用抗小鼠 IgM 单抗包被于酶标板上,标准品和样品中的 IgM 与单抗结合,加入酶标抗体,形成免疫复合物连接在板上,加入酶底物TMB ,出现蓝色,加终止液硫酸,颜色变黄,在 4 50 nm 处测 OD
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 大鼠 IgM 定量 EIA 试剂盒使用说明 原理 本实验采用双抗体夹心 ELISA 法。用抗大鼠 IgM 单抗包被于酶标板上,标准品和样品中的 IgM 与单抗结合,加入酶标抗体,形成免疫复合物连接在板上,加入酶底物 TMB ,出现蓝色,加终止液硫酸,颜色变黄,在 450nm 处测 OD 值
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