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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20˚C
- 英文名:
Recombinant Porcine circovirus 2 Capsid protein(Cap)
- 库存:
详询
- 供应商:
南京莱富赛
- 规格:
详询
host_species:E.coli
purification:Greater than 85% as determined by SDS-PAGE.
other_names:
accession_no:O56129
calculated_mw:47.9 kDa
tag info:N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
Immunogen Description:Expression Region:1-233aa
Sequence Info:Full Length
formulation:Tris-based buffer50% glycerol
storage:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃,-80℃. The shelf life of lyophilized form is 12 months at -20℃,-80℃.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
background:Self-assembles to form the virion icosahedral capsid with a T=1 symmetry. This very small capsid (17 - 22 nm in diameter) allows the virus to be very stable in the environment and resistant to some disinfectants, including detergents. Essential for the initial attachment to heparan sulfate moities and chondroitin sulfate B of the host cell surface proteoglycans. After attachment, the virus is internalized in a clathrin-, caveolae- and dynamin-independent, actin and Rho-GTPase-mediated pathway and traffics to the nucleus. The capsid protein binds and transports the viral genome and Rep across the nuclear envelope
产品详细信息请点击:Recombinant Porcine circovirus 2 Capsid protein(Cap)
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文献和实验genes can be deleted except for two short regions. The first one encompasses 32 amino acid (aa) residues from the N-terminal coding sequence of capsid (C) and the second, 27 aa region from the C-terminus of envelope (E) protein. The deleted region
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
. Gelatin/sucrose embedding gives better frozen sections for embryonic tissue than does OCT. Gelatin/sucrose embedding solution 7.5% gelatin (porcine skin, Sigma) 15% sucrose 0.05% sodium azide in 1X PBS Dissolve
):单倍体基因组中DNA 的总量。 CAAT box (CAAT 盒):真核生物转录单位起始点上游的保守序列,被一组转录因子识别。 Cap (帽):是真核生物mRNA 5‘端的结构,在转录后通过末端5‘GTP的磷酸基团和mRNA的末端碱基而引入。增加的G(有时是其它碱基)是甲基化的,产生了MeG5‘pppNp… 的结构。 CAP(CRP):由cAMP 激活的正调控蛋白质。对RNA 聚合酶起始E.coli 中一些操纵子(分解代谢——敏感)是必须的。 Capsid (衣壳):是病毒微粒外部
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