- 询价
- Signalway Antibody
- USA
- AP77306
- 2025年07月08日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Store at -20˚C
- 英文名：
Recombinant Influenza A virus Matrix protein 1(M)
- 库存：
详询
- 供应商：
南京莱富赛
- 规格：
详询
host_species:E.coli
purification:Greater than 85% as determined by SDS-PAGE.
other_names:
accession_no:A4GCL0
calculated_mw:32.8 kDa
tag info:N-terminal 10xHis-tagged and C-terminal Myc-tagged
Immunogen Description:Expression Region:1-252aa
Sequence Info:Full Length
formulation:Tris-based buffer50% glycerol
storage:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃,-80℃. The shelf life of lyophilized form is 12 months at -20℃,-80℃.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
background:Plays critical roles in virus replication, from virus entry and uncoating to assembly and budding of the virus particle. M1 binding to ribonucleocapsids (RNPs) in nucleus seems to inhibit viral transcription. Interaction of viral NEP with M1-RNP is thought to promote nuclear export of the complex, which is targeted to the virion assembly site at the apical plasma membrane in polarized epithelial cells. Interactions with NA and HA may bring M1, a non-raft-associated protein, into lipid rafts. Forms a continuous shell on the inner side of the lipid bilayer in virion, where it binds the RNP. During virus entry into cell, the M2 ion channel acidifies the internal virion core, inducing M1 dissociation from the RNP. M1-free RNPs are transported to the nucleus, where viral transcription and replication can take place Determines the virion's shape: spherical or filamentous. Clinical isolates of influenza are characterized by the presence of significant proportion of filamentous virions, whereas after multiple passage on eggs or cell culture, virions have only spherical morphology. Filamentous virions are thought to be important to infect neighboring cells, and spherical virions more suited to spread through aerosol between hosts organisms
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文献和实验Influenza A Virus Molecular Virology Techniques
influenza A viruses by plasmid-based reverse genetics. To accelerate the amplification and cloning process, we developed multi-segment-RTPCR (M-RTPCR) techniques that efficiently amplify the eight genomic viral RNA segments (vRNAs) of influenza A virus
An Immunoproteomics Approach to Screen the Antigenicity of the Influenza Virus
The structure and antigenicity of protein antigens of the influenza virus are screened in a single step employing an immunoproteomics approach. Matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) coupled to gel
PCR for the Detection of Influenza Viruses in Clinical Material
Influenza viruses are segmented, negative-stranded RNA viruses belonging to the family Orthomyxoviridae . They are classified into influenza A, B, and C on the basis of different epitopes on the nucleoprotein (NP) and matrix proteins (M
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