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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20˚C
- 英文名:
Recombinant Human C-X-C Motif Chemokine 14,CXCL14
- 库存:
详询
- 供应商:
南京莱富赛
- 规格:
详询
host_species:E.coli
purification:Greater than 95% as determined by reducing SDS-PAGE.
specificity:Human
accession_no:O95715
calculated_mw:9.4kDa
formulation:Lyophilized from a 0.2 μm filtered solution of 20mM TrisHCl, 1M NaCl, pH 8.5.
reconstitution:Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg,ml.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
storage:Lyophilized protein should be stored at < -20C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20C for 3 months.
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文献和实验Mutational Analysis of the Human Protein C Gene
The single gene for protein C is located at position q13–q14 on chromosome 2 (1 ). Two groups have described human genomic clones of protein C isolated from phage l charon libraries using cDNA for human protein C as hybridization probes
Introduction of human 14-3-3 proteins
whose members have been shown to activate tyrosine and tryptophan hydroxylases,inhibit protein kinase C and possess phospholipase A2 activity in vitro.We have isolated and analyzed a 14-3-3 protein cDNA clone (H14-3-3)from a human fetal brain cDNA library
A/B/C Random Amplification Protocol
tubes. Add 1 µL DNA (40 ng) to each tube. Digest DNA then heat inactivate enzyme on PCR machine: ( 37ºC x60', 65ºC x10') x1 (4ºC hold) 4. While the genomic DNA is digesting set up master mixes for Reactions I, II, and III
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