- 询价
- Signalway Antibody
- 48894
- 2025年09月23日
- WB, ICC/IF, IHC
- Recombinant Rabbit
- Human;Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
recombinant protein
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Monoclonal antibody
- 适应物种：
Human;Mouse
- 保质期：
12 months
- 抗原来源：
Recombinant Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Recombinant Rabbit
- 应用范围：
WB, ICC/IF, IHC
- 靶点：
Uniprot：P11309
- 抗体英文名：
PIM1 Rabbit mAb
- 规格：
50ul/100ul
Oncogene PIM1 antibody
PIM 1 antibody
pim 1 kinase 44 kDa isoform antibody
Pim 1 kinase antibody
pim 1 oncogene (proviral integration site 1) antibody
Pim 1 oncogene antibody
PIM antibody
PIM1 antibody
pim1 kinase 44 kDa isoform antibody
PIM1_HUMAN antibody
Pim2 antibody
PIM3 antibody
Proto oncogene serine/threonine protein kinase Pim 1 antibody
Proto-oncogene serine/threonine-protein kinase Pim-1 antibody
Proviral integration site 1 antibody
Proviral integration site 2 antibody
计算分子量:32 kDa
配方:1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
应用详情:WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200
图片:
Western blot analysis of PIM1 on different lysates using anti-PIM1 antibody at 1/1,000 dilution. Positive control:
Lane 1: BT-20
Lane 2: HepG2
Lane 3: SW480
,
Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-PIM1 antibody. Counter stained with hematoxylin.
,
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PIM1 antibody. Counter stained with hematoxylin.
,
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PIM1 antibody. Counter stained with hematoxylin.
,
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PIM1 antibody. Counter stained with hematoxylin.
,
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PIM1 antibody. Counter stained with hematoxylin.
,
Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-PIM1 antibody. Counter stained with hematoxylin.
,
ICC staining PIM1 in BT-20 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
,
ICC staining PIM1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
,
ICC staining PIM1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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文献和实验：使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。（图 A）使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit，抗体 1:100 稀释；(图 B) 采用普通免疫组化试剂盒，抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注：使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK （Erk1/2）Rabbit mAb 对正常小鼠心脏组织进行免疫
h）即可。2. 修复大法——不仅仅是「煮一煮」微波炉修复：简单易行效果好，CST 推荐使用微波炉完成修复。合适的修复液：根据抗体说明书使用合适的修复液。用柠檬酸修复后，切片需浸泡在修复液中，自然冷却；而用 EDTA 修复后，切片可直接从修复缸中取出，直接进行下一步。注：使用不同的修复方式和不同生产商的抗体检测人肺癌组织中 EGFR 的表达。第一排为 CST 的 EGF Receptor (D38B1) XP® Rabbit mAb（#4267），EDTA 的修复方式明显优于柠檬酸盐及胃蛋白
到 PVDF 膜上，同时减少蛋白不必要降解，这对于最终获得清晰、可信结果也是需要考虑因素。抗体信息：1.ACC1, Recombinant Rabbit monoclonal IgG. HuaAn. HuaAn biotechnology , inc.2.ATGL, Mouse mAb IgG1, Cat#:RT1058. HuaAn biotechnology , inc.3.p-PERK(Thr981), Rabbit Polyclonal IgG primary antibodies, Cat
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