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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
A synthesized peptide derived from human RIPK1 around the phosphorylation site of Tyr384
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Polyclonal
- 适应物种:
Hu, Ms, Rt
- 宿主:
Rabbit
- 应用范围:
WB
- 浓度:
1mg/ml
- 抗体英文名:
RIPK1 (Phospho-Tyr384) Antibody
clonality:Polyclonal
specificity:Phospho-RIPK1(Phospho-Tyr384) Antibody detects endogenous levels of RIPK1 only when phosphorylated at Tyr384
other_names:Cell death protein RIP; FLJ39204; OTTHUMP00000039163; Receptor (TNFRSF) interacting serine threonine kinase 1; receptor interacting protein 1; Receptor interacting protein; Receptor interacting protein kinase 1; Receptor interacting serine threonine protein kinase 1; Receptor TNFRSF interacting serine threonine kinase 1; Receptor-interacting protein 1; Receptor-interacting serine/threonine-protein kinase 1; Rinp; RIP 1; RIP; Rip-1; RIP1; RIPK 1; Ripk1; RIPK1_HUMAN; Serine threonine protein kinase RIP; Serine/threonine-protein kinase RIP; accession_no:Swiss-Prot#:Q13546
NCBI Gene ID:8737
产品详细信息请点击:RIPK1 (Phospho-Tyr384) Antibody
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文献和实验largely independent of the surrounding amino-acid sequence. The antibody is phospho-specific but does not cross-react with phospho-serine-containing sequences. The anti-phosphothreonine monoclonal antibody is labeled with Eu3+ Cryptate by Cisbio
运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白
using Phospho-p38 antibody (A) and Total-p38 antibody (B). Cell Based Elisa were performed directly in the 96-well plates using Phospho-p38 and Total-p38 antibodies (C). Figure 3: JNK detection with FACECells were serum-starved for 16 hours
。所以,我们建议使用微波炉或高压锅加热煮沸以达到最佳抗原修复效果。抗体:Phospho–Stat3 (Tyr705)(D3A7) XP. Rabbit mAb #9145样本:石蜡包埋人类肺部肿瘤样本煮沸设备:浴锅 (左),微波炉 (中),高压锅 (右)免疫染色—封闭在 IHC–P 中,我们建议在室温下在含有 Tween20 的 TBST 缓冲液和 5% 正常山羊血清 (NGS) 中封闭样品,时长 1 小时,以防止非特异性背景染色。在进行 IHC–F 时,在含有 0.3% Triton. X–100 的 1X
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