Anti-IPO7 antibody

Anti-IPO7 antibody

Cat. No.     JC261794
Package    25 μl/100 μl/200 μl
Storage    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Product overview
Description                   Anti-IPO7 rabbit polyclonal antibody
Applications        ELISA, WB, IHC 
Immunogen               Synthetic peptide of human IPO7
Reactivity                 Human, Mouse
Content                     0.5 mg/ml
Host species               Rabbit
Ig class                  Immunogen-specific rabbit IgG
Purification                 Antigen affinity purification

Target information
Symbol                 IPO7
Full name                 importin 7
Synonyms    Imp7; RANBP7
Swissprot    O95373

Target Background
The importin-alpha/beta complex and the GTPase Ran mediate nuclear import of proteins with a classical nuclear localization signal. The protein encoded by this gene is a member of a class of approximately 20 potential Ran targets that share a sequence motif related to the Ran-binding site of importin-beta. Similar to importin-beta, this protein prevents the activation of Ran's GTPase by RanGAP1 and inhibits nucleotide exchange on RanGTP, and also binds directly to nuclear pore complexes where it competes for binding sites with importin-beta and transportin. This protein has a Ran-dependent transport cycle and it can cross the nuclear envelope rapidly and in both directions. At least four importin beta-like transport receptors, namely importin beta itself, transportin, RanBP5 and RanBP7, directly bind and import ribosomal proteins.

Applications
Immunohistochemistry
Predicted cell location: Cytoplasm or Nucleus
Positive control: Human gasrtic cancer
Recommended dilution: 100-300    Predicted cell location: Cytoplasm or Nucleus
Positive control: Human liver cancer
Recommended dilution: 100-300
            
The image on the left is immunohistochemistry of paraffin-embedded Human gasrtic cancer tissue using ml261794(IPO7 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200)    The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using ml261794(IPO7 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200)
Western blotting
Predicted band size:120 kDa
Positive control:Hela cells 
Recommended dilution: 500-2000