Product overview
Description Anti-NEK4 rabbit polyclonal antibody
Applications ELISA, IHC
Immunogen Synthetic peptide of human NEK4
Reactivity Human, Mouse
Content 0.1 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol NEK4
Full name NIMA-related kinase 4
Synonyms NRK2; STK2; pp12301
Swissprot P51957
Target Background
NIMA was originally shown in Aspergillus nidulans to be necessary for entry into mitosis. NIMA-related mammalian proteins have since been identified as Nek1, Nek2, Nek3 and Nek4 (also designated STK2 or NRK2). High expression of Nek1 is seen in male and female germ cell lines of mouse. Nek2 is the closest known mammalian relative to NIMA. Like NIMA, Nek2 expression peaks at the G2 to M phase transition. Nek3 is a predominantly cytoplasmic enzyme that was detectable in all organs studied. Levels of Nek3 seem to remain unchanged throughout the cell cycle, but appear to be elevated in G0-arrested, quiescent fibroblasts. In developing testicular germ cells, differential patterns of expression were seen for Nek1, Nek2 and Nek4, indicating possible overlapping, but non-identical functions.
Applications
Immunohistochemistry
Predicted cell location: Nucleus
Positive control: Human cervical cancer
Recommended dilution: 25-100 Predicted cell location: Nucleus
Positive control: Human lung cancer
Recommended dilution: 25-100
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using ml261378(NEK4 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using ml261378(NEK4 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200)