品牌:CST,产品名称:PEA-15 (D1G6A) Rabbit mAb #13091 英文名称:PEA-15 (D1G6A) Rabbit mAb Product name:PEA-15 (D1G6A) Rabbit mAb #13091 Source:Rabbit Sensitivity:Endogenous MW(kDa):15 Applications:WB , IP , Purification: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu60 of human PEA-15 protein. Background: PEA-15 is a 15 kDa phosphoprotein expressed abundantly in astrocytes and fibroblasts as well as in tissues, including the lung and eye (1). The protein has been shown to coordinate cell growth, death, and glucose utilization (2-4). The amino-terminal DED domain of PEA-15 mediates its binding to FADD or Erk and further regulates the Erk and apoptosis signaling pathways. PEA-15 can be phosphorylated at two serine residues, Ser104 and Ser116, located within the carboxy terminus. Phosphorylation at these sites regulates binding to Erk and FADD (2,3).
PEA-15 (D1G6A) Rabbit mAb recognizes endogenous levels of total PEA-15 protein.
Species Reactivity:
Human, Mouse, Rat
Application
Dilutions
Western Blotting
1:1000
Immunoprecipitation
1:50
Storage:
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.