品牌:CST,产品名称:APPL1 (D83H4) XP Rabbit mAb #3858 英文名称:APPL1 (D83H4) XP® Rabbit mAb Product name:APPL1 (D83H4) XP® Rabbit mAb #3858 Isotype:Rabbit IgG Sensitivity:Endogenous MW(kDa):82 Reactivity:H,M,R,Mk Applications:WB , IP , IF , Purification: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr426 of human APPL1. Background: The APPL1 multidomain adaptor protein is a BAR-domain protein family member that is involved in membrane trafficking within a number of signal transduction pathways (1). The amino-terminal BAR domain mediates the formation of crescent-shaped APPL1 homodimers (or APPL1 and APPL2 heterodimers) important to lipid binding and membrane curvature sensing (1). The PH domain of APPL1 is required for binding of the adaptor protein to Rab5 GTPase (2). In response to extracellular stimuli, Rab5 GTP hydrolysis releases APPL1 from the endosome and allows translocation of APPL1 to the nucleus where it joins a protein complex that controls chromatin remodeling and gene expression (3). The carboxy-terminal PTB domain of APPL1 enables an interaction between APPL1 and the TrkA neurotrophin receptor. An association between these two proteins and the TrkA-interacting protein GIPC1 within endosomes is required for nerve growth factor mediated signaling (4). APPL1 also binds follicle-stimulating hormone (FSH) receptors, which may provide a relay of FSH signaling to the PI3K/Akt pathway (5). The APPL1 adaptor protein is implicated in insulin signaling, as interaction between APPL1 and Akt2 is required for insulin-stimulated translocation of GLUT4 receptor proteins. Both induced overexpression and knockdown of APPL1 inhibit insulin-stimulated GLUT4 translocation (6). APPL1 binds the adiponectin receptor and acts as a downstream effector in the adiponectin pathway to mediate NO production (7,8). APPL1 interacts with DCC (deleted in colorectal cancer) protein and may play a role in DCC-induced apoptosis (9).
APPL1 (D83H4) XP® Rabbit mAb detects endogenous levels of total APPL1 protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Application
Dilutions
Western Blotting
1:2000
Immunoprecipitation
1:100
Immunofluorescence (Immunocytochemistry)
1:200
Storage:
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.