品牌:CST,产品名称:Miwi (D92B7) XPRabbit mAb #6915 英文名称:Miwi (D92B7) XP® Rabbit mAb Product name:Miwi (D92B7) XP® Rabbit mAb #6915 Isotype:Rabbit IgG Sensitivity:Endogenous MW(kDa):80 Applications:WB , IP , IF , Purification: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly81 of mouse Miwi protein. Background: The Drosophila piwi gene was identified as being required for the self-renewal of germline stem cells (1). Piwi homologs are well conserved among various species including Arabidopsis, C. elegans, and Homo sapiens (1). Both Miwi and Mili proteins are mouse homologs of Piwi and contain a C-terminal Piwi domain (2). Miwi and Mili bind to Piwi-interacting RNAs (piRNAs) in male germ cells and are essential for spermatogenesis in mice (3-5).
Miwi (D92B7) XP® Rabbit mAb recognizes endogenous levels of total Miwi protein.
Species Reactivity:
Mouse
Species predicted to react based on 100% sequence homology:
Human, Rat
Application
Dilutions
Western Blotting
1:1000
Immunoprecipitation
1:50
Immunofluorescence (Frozen)
1:800
Storage:
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.