品牌:CST,产品名称:PDE4B (D7R1Y) Rabbit mAb #72096 英文名称:PDE4B (D7R1Y) Rabbit mAb Product name:PDE4B (D7R1Y) Rabbit mAb #72096 Isotype:Rabbit IgG Sensitivity:Endogenous MW(kDa):60 Applications:WB , IP , Purification: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu61 of human PDE4B protein. Background: Enzymes of the phosphodiesterase (PDE) superfamily catalyze the hydrolysis of 3',5'-cyclic nucleotides into the corresponding nucleotide 5'-monophosphates. The PDE superfamily includes 11 subfamilies (PDE1-PDE11) in mammals (1). These enzymes function as important positive and negative regulators of cellular response, including regulation of insulin secretion, heart function, erectile function, and inflammation (2-5). The cAMP-specific phosphodiesterase 4B (PDE4B, DPDE4) is important for the inflammatory response to lipopolysaccharide in monocytes (6). PDE4B plays an important role in the hydrolysis and inactivation of the ubiquitous second messenger cAMP that regulates lymphocyte cell growth and apoptosis (7). Research studies indicate that PDE4B is also involved in psychiatric disorders, including schizophrenia, autism, and depression (8-10).
PDE4B (D7R1Y) Rabbit mAb recognizes endogenous levels of total PDE4B protein. This antibody does not cross-react with PDE4A protein.
Species Reactivity:
Human, Mouse, Rat
Application
Dilutions
Western Blotting
1:1000
Immunoprecipitation
1:50
Storage:
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.