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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human full-length CD86 protein
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX34570
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
ICC/IF, IHC-P
- 浓度:
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
CD86
- 抗体英文名:
CD86 antibody [SPM600]
- 抗体名:
CD86 抗体 [SPM600]
- 规格:
100 μg
IHC-P analysis of human melanoma tissue using GTX34570 CD86 antibody [SPM600].
ICC/IF analysis of Ramos cells using GTX34570 CD86 antibody [SPM600].
Green : Primary antibody
Red : nucleus
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文献和实验for this purpose. Grow cells in 200 mls of YPD at 30oC to an OD 600 of 1. Add formaldehyde to the 200 ml culture to a final concentration of 3.7. Fix for 1 hour at room temperature with shaking. Centrifuge cells at 3000 X g. Resuspend
Antibody cleanup In order to decrease the amount of nonspecific staining, it is often necessary to preabsorb primary and secondary antibodies to yeast cells lacking the antigen prior to use. A 1:1 mixture of fixed yeast
Antibody Staining of Ovaries 1. Dissect ovaries in EBR 2. Transfer ovaries into an Eppendorf tube and add 100 µl devitellinizing buffer and 600-µl heptane 3. Agitate gently for 10 minutes
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