- ￥5300
- GeneTex
- 美国
- GTX17513
- 2025年10月14日
- WB, ICC/IF
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Peptide sequence around phosphorylation site of serine 15 (A-N-S(p)-N-V) derived from human Myosin Light Chain 2 (MLC-2v).
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX17513
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Myosin Light Chain 2 (MLC-2v) (phospho Ser15)
- 抗体英文名：
Myosin Light Chain 2 (MLC-2v) (phospho Ser15) antibody
- 抗体名：
Myosin Light Chain 2 (MLC-2v) (phospho Ser15) 抗体
- 规格：
100 μl
WB analysis of extracts from U87 cells synchronized in interphase ( I ) and cytokinesis ( C ) using GTX17513 Myosin Light Chain 2 (MLC-2v) (phospho Ser15) antibody.
ICC/IF analysis of methanol-fixed U87 cells using GTX17513 Myosin Light Chain 2 (MLC-2v) (phospho Ser15) antibody.
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文献和实验Myosin Light Chain Preparation
in lyophilizer bottles and freeze in liquid nitrogen.Put on lyophilizer and leave until dry (overnight or over weekend). Day 5 22.Resuspend separate light chain pools 1 and 2 in a small volume of Dialyzing Solution G.Pipette into dialyzing tubing (size #20
Purification of Myosin Light Chain Kinase
RPM (4℃).Filter the supernatant through glass wool and discard the pellet (or save for light chain prep).Save 1 ml of supernatant (4℃)for assay.Add pepstatin and TLCK to final concentrations of 1 μg/ml and 40 Og/ml,respectively. Volume of supernatant
Labeling Gizzard Myosin with Tetramethylrhodamine Iodoacetamide (Primarily 17kDa Light Chain)
pellet in a small volume of buffer 1 to obtain 15-20 mg/ml concentration.Dialyze against 500 ml of buffer 1 for >=2 hours. 4.Measure volume.Dilute with dialysis buffer to obtain a myosin solution of 12-15 mg/ml. 5.Get an aliquot of IATR,dissolve in DMF
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