Sandwich ELISA detection of beta Amyloid (1-42)–peptide aggregate using GTX635160 as capture antibody at concentration of 10 μg/mL and GTX134510 as detection antibody at concentration of 0.45 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:2000 and used to detect the primary antibody.
ELISA plate is coated with 100 μL of beta Amyloid (1-42) peptide aggregate at 10 μg/mL. The coated protein is detected with beta Amyloid (1-42) antibody – Conformation Specific (GTX134510) at concentrations ranging from 45 ng/mL down to 1.67 ng/mL.
Beta amyloid (1-42) antibody (conformation specific) detects beta amyloid protein aggregates by immunohistochemical analysis in the hippocampus of 3xTg-AD transgenic mouse brain (left), but not in age- and gender- matched normal mouse brain (right).Sample: Paraffin-embedded 3xTg-AD transgenic mouse brain (left) and healthy mouse brain (right).beta Amyloid (1-42) stained by beta Amyloid (1-42) antibody – Conformation Specific (GTX134510) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0
Conformation-specific beta amyloid antibody detects beta amyloid protein aggregates in the occipital lobe of human Alzheimer's disease brain by immunohistochemical analysis.Antibodies: beta amyloid (1-42) antibody – Conformation Specific (GTX134510) diluted at 1:500, and competitor's antibody diluted at 1:500.
*Competitor's antibody is not affiliated with GeneTex and does not endorse this product.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Beta amyloid (1-42) antibody (conformation specific) detects beta amyloid protein aggregates by immunohistochemical analysis in the occipital lobe of Alzheimer's disease brain (left), but not in normal human brain(right).Sample: Paraffin-embedded Human Brain (Alzheimer's Disease + Normal) tissue slides (GTX49382).beta amyloid protein aggregates stained by beta amyloid (1-42) antibody – Conformation Specific (GTX134510) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Dot blot analysis of 200ng beta amyloid peptide (1-42) oligomers or monomers, using Beta amyloid (1-42) antibody – Conformation Specific (GTX134510) diluted at 1:4000, 1:8000, and 1:16000.
Conformation-specific beta amyloid antibody detects beta amyloid protein aggregates in the postcentral gyrus of human Alzheimer's disease brain by immunohistochemical analysis.Antibodies: beta amyloid (1-42) antibody – Conformation Specific (GTX134510) diluted at 1:500, and competitor's antibody diluted at 1:500.
*Competitor's antibody is not affiliated with GeneTex and does not endorse this product.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min