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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human TGF beta Receptor I. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX134209
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB
- 浓度:
2.39 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
TGF beta Receptor I
- 抗体英文名:
TGF beta Receptor I antibody
- 抗体名:
TGF beta Receptor I 抗体
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
HepG2 whole cell and cytoplasmic extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with TGF beta Receptor I antibody (GTX134209) diluted at 1:1000 and competitor's antibody (ab31013) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
A549 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with TGF beta Receptor I antibody (GTX134209) diluted at 1:1000 and competitor's antibody (ab31013) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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文献和实验Chiou WC et al., Biomed Pharmacother 2023 (PMID:36906971)
Hsu WH et al., Int J Biol Macromol 2020 (PMID:33011264)
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
人转化生长因子-b2(TGF-b2)ELISA试剂盒 说明书
倍数 1 0 。 ( 注意:不同的标本TGF-β 2 的水平可能有较大差异,请根据实际情况灵活掌握稀释度) 检测程序 1. 加样:每孔各加入标准品或待测样品(已激活) 100ul ,将反应板充分混匀后置 37 ℃ 120 分钟。 2. 洗板:用洗涤液将反应板充分洗涤 4-6 次,向滤纸上印干。 3. 每孔中加入第一抗体工作液10 0ul 。将反应板充分混匀后置 37 ℃ 6 0 分钟。 4. 洗板:同前。 5. 每孔加
1.5ml 进口聚丙烯管中 , 再加 10ul 血清或血浆标本。 2. 加20ul 1 N HC I ,盖紧,上下混匀。 2 - 8 ℃ 放置60± 2 分钟。 3. 加20ul 1 N NaOH, 盖紧,上下混匀。 4. 即用,或放-20/-70 ℃ 保存3 天。计算结果时乘 以稀释倍数 50 。 ( 注意:不同的标本TGF-β 的水平可能有较大差异,请根据实际情况灵活掌握稀释度)
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