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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
A synthetic peptide corresponding to a sequence in the middle region of human NOX2(442-459aa YWLCRDTHAFEWFADLLQ), identical to the related rat and mouse sequences.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX12024
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, IHC-P
- 浓度:
500 μg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
NOX2 / gp91phox
- 抗体英文名:
NOX2 / gp91phox antibody
- 抗体名:
NOX2 / gp91phox 抗体
- 规格:
100 μg
IHC-P analysis of human intestinal cancer tissue using GTX12024 NOX2 / gp91phox antibody.
WB analysis of various samples using GTX12024 NOX2 / gp91phox antibody.
Lane 1 : rat thymus tissue lysates
Lane 2 : rat brain tissue lysates
Dilution : 0.5 μg/mL
Loading : 50μg of sample under reducing conditions
WB analysis of various samples using GTX12024 NOX2 / gp91phox antibody.
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : MCF-7 cell lysate
Lane 4 : SMMC cell lysate
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文献和实验Wei X et al., Medicine (Baltimore) 2022 (PMID:35960091)
Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
Although Y3/Ag1.2.3 has its own myeloma light chain, their are numerous characteristics that make this a better parent line for making hybridomas than the 'Y0' chain loss variants or using NS0 to make rat x mouse hybrids. These are: a) Hybridomas
There is an increasing interest in the preparation of rat � rat hybridomas, because they have been found to be more stable in culture than mouse hybridomas and they secrete consistently high levels (10 �g/mL and above) of monoclonal antibody
人转录因子E2F1 ( E2F1 )ELISA 试剂盒 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 E2F1 单抗包被于酶标板上,标准品和样品中的 E2F1与单抗结合,加入生物素化的抗人 E2F1 ,形成免疫复合物连接在板上,辣根过氧化物酶标记的 Streptavidin 与生物素结合,加入底物工作液显蓝色,最后加终止液硫酸,在 450nm 处测 OD 值,E2F1 浓度与 OD 值成正比
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