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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海研卉生物科技有限公司
- 库存：
25T
- 级别：
科研级别，不能用于临床和体外诊断
- 目录编号：
305103
- 克隆性：
单克隆
- 抗体英文名：
FITC anti-human CD66b
- 抗体名：
FITC anti-human CD66b
- 标记物：
FITC
- 宿主：
小鼠
- 适应物种：
人
- 亚型：
Mouse IgM, κ
- 应用范围：
流式
- 规格：
25T
- Workshop
- VI MA81
- Other Names
- CD67, CGM6, NCA-95, CEACAM8
- Isotype
-
Mouse IgM, κ
DescriptionCD66b is a 95-100 kD glycosylphosphatidylinositol (GPI)-linked protein also known as CD67, CGM6, and NCA-95. CD66b is a member of the immunoglobulin superfamily, carcinoembryonic antigen (CEA)-like subfamily. CD66b, expressed on granulocytes, has been reported to induce activation in neutrophils and to be involved in heterophilic adhesion with CD66c.
- Isotype Control
- FITC Mouse IgM, κ Isotype Ctrl
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
- Blue Laser (488 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunohistochemical staining of ac etone-fixed frozen, formalin-fixed paraffin-embedded tissue sections, and spatial biology (IBEX)5,6.
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文献和实验- Application References
(PubMed link indicates BioLegend citation) -
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
- Norling LV, et al. 2012. Arterioscler Thromb Vasc Biol. 32:1970. PubMed
- Meinke P, et al. 2015. Neuroimmunol Discord. 25:127. PubMed
- Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- Product Citations
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CD66a/CD66b/CD66c/CD66d/CD66e/CD66f/CD67分子
CD66a/CD66b/CD66c/CD66d/CD66e/CD66f/CD67 分子 CD66a 常用单克隆抗体或代号： BGP- 1 主要表达细胞： G,En [M] 分子质量(kDa)和结构： gp180- 200(胆汁糖蛋白- 1)(IgSF) 功 能： 嗜同性结合,也可识别CD62E CD66b 常用单克隆抗体或代号： MF25.1;(P100,原CD67,CGM6) 主要表达
FITC荧光二抗？――EarthOx新型DyLight 488绿色荧光
FITC荧光二抗？――EarthOx新型DyLight 488绿色荧光 FITC（Fluorescein Isothiocyanate，异硫*酸荧光素）是一种绿色荧光团，在免疫学实验里，经常被用于荧光团标记到相应的检测分子，如抗体上。FITC的纯品为黄色或橙黄色结晶粉末，易溶于水和酒精溶剂。FITC分子量为389.4，最大吸收光波长为 490～495nm，最大发射光波长为520～530nm，呈现明亮的黄绿色荧光。FITC在冷暗干燥处可保存多年，是目前应用最广泛的荧
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