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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 供应商:
江西江蓝纯生物试剂有限公司
- 库存:
大量
- 克隆性:
多克隆
- 保质期:
1年
- 抗体英文名:
Anti-CD63 antibody
- 宿主:
Rabbit
- 浓度:
0.5 mg/ml
- 保存条件:
-20°C
- 规格:
25 μl/100 μl/200 μl
Cat. No. JLC260973
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-CD63 rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Synthetic peptide of human CD63
Reactivity Human
Content 0.5 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol CD63
Full name CD63 molecule
Synonyms MLA1; ME491; LAMP-3; OMA81H; TSPAN30
Swissprot P08962
Target Background
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms.

Applications
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human liver cancer
Recommended dilution: 25-100 Predicted cell location: Cytoplasm
Positive control: Human brain
Recommended dilution: 25-100
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using ml260973(CD63 Antibody) at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using ml260973(CD63 Antibody) at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: ×200)
Western blotting
Predicted band size:26 kDa
Positive control:Human bladder carcinoma tissue
Recommended dilution: 200-1000
Gel: 12%SDS-PAGE
Lysate: 40 μg
Lane: Human bladder carcinoma tissue
Primary antibody: ml260973(CD63 Antibody) at dilution 1/200
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 2 minutes
ELISA
Recommended dilution: 1000-2000
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