Product overview Description Anti-RBMS3 rabbit polyclonal antibody Applications ELISA, WB, IHC Immunogen Synthetic peptide of human RBMS3 Reactivity Human, Mouse Content 0.5 mg/ml Host species Rabbit Ig class Immunogen-specific rabbit IgG Purification Antigen affinity purification
Target information Symbol RBMS3 Full name RNA binding motif, single stranded interacting protein 3 Synonyms Swissprot Q6XE24
Target Background This gene encodes an RNA-binding protein that belongs to the c-myc gene single-strand binding protein family. These proteins are characterized by the presence of two sets of ribonucleoprotein consensus sequence (RNP-CS) that contain conserved motifs, RNP1 and RNP2, originally described in RNA binding proteins, and required for DNA binding. These proteins have been implicated in such diverse functions as DNA replication, gene transcription, cell cycle progression and apoptosis. The encoded protein was isolated by virtue of its binding to an upstream element of the alpha2(I) collagen promoter. The observation that this protein localizes mostly in the cytoplasm suggests that it may be involved in a cytoplasmic function such as controlling RNA metabolism, rather than transcription. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Applications Immunohistochemistry Predicted cell location: Cytoplasm Positive control: Human thyroid cancer Recommended dilution: 50-100 Predicted cell location: Cytoplasm Positive control: Human liver cancer Recommended dilution: 50-100
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using ml260728(RBMS3 Antibody) at dilution 1/70, on the right is treated with synthetic peptide. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using ml260728(RBMS3 Antibody) at dilution 1/70, on the right is treated with synthetic peptide. (Original magnification: ×200) Western blotting Predicted band size:48 kDa Positive control:Human liver cancer tissue Recommended dilution: 1000-5000
Gel: 10%SDS-PAGE Lysate: 40 μg Lane: Human liver cancer tissue Primary antibody: ml260728(RBMS3 Antibody) at dilution 1/1000 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 20 seconds