- 询价
- gelatins
- JLC260599
- 上海
- 2025年07月16日
- Rabbit
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- 详细信息
- 技术资料
- 供应商：
江西江蓝纯生物试剂有限公司
- 库存：
大量
- 克隆性：
多克隆
- 保质期：
1年
- 抗体英文名：
Anti-MCM4 antibody
- 宿主：
Rabbit
- 浓度：
0.3 mg/ml
- 保存条件：
-20°C
- 规格：
25 μl/100 μl/200 μl
Anti-MCM4 antibody
Cat. No. JLC260599
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-MCM4 rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Synthetic peptide of human MCM4
Reactivity Human, Mouse
Content 0.3 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol MCM4
Full name Minichromosome maintenance complex component 4
Synonyms CDC21, CDC54, hCdc21, P1-CDC21
Swissprot P33991
Target Background
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported.
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm, Nucleus
Positive control: Human brain
Recommended dilution: 50-200 Predicted cell location: Cytoplasm, Nucleus
Positive control: Human breast cancer
Recommended dilution: 50-200
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using ml260599(MCM4 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using ml260599(MCM4 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200)
Western blotting
Predicted band size:97 kDa
Positive control:NIH/3T3, Hela and K562 cells
Recommended dilution: 500-2000
Gel: 8%SDS-PAGE
Lysate: 50 μg
Lane 1-3: NIH/3T3 cells, Hela cells, K562 cells
Primary antibody: ml260599(MCM4 Antibody) at dilution 1/700
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 10 seconds
ELISA
Recommended dilution: 1000-5000
Cat. No. JLC260599
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-MCM4 rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Synthetic peptide of human MCM4
Reactivity Human, Mouse
Content 0.3 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol MCM4
Full name Minichromosome maintenance complex component 4
Synonyms CDC21, CDC54, hCdc21, P1-CDC21
Swissprot P33991
Target Background
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported.
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm, Nucleus
Positive control: Human brain
Recommended dilution: 50-200 Predicted cell location: Cytoplasm, Nucleus
Positive control: Human breast cancer
Recommended dilution: 50-200
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using ml260599(MCM4 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using ml260599(MCM4 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200)
Western blotting
Predicted band size:97 kDa
Positive control:NIH/3T3, Hela and K562 cells
Recommended dilution: 500-2000
Gel: 8%SDS-PAGE
Lysate: 50 μg
Lane 1-3: NIH/3T3 cells, Hela cells, K562 cells
Primary antibody: ml260599(MCM4 Antibody) at dilution 1/700
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 10 seconds
ELISA
Recommended dilution: 1000-5000
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Anti-MCM4 antibody
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