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文献和实验CD3/ CD4 /CD8 T cell subset counts
the sample ID, test to be performed and date. 2. 15 ƒY´l of the Test antibody (CD3-FITC / CD8-PE / CD4-PECy5) (Cytognos) is added to each sample tube. 3. 100 ƒY´l of blood sample is added to corresponding test tube by REVERSE PIPETTING and the tubes
CD3/ CD4 /CD8 T cell subset counts
the sample ID, test to be performed and date. 2. 15 ƒY´l of the Test antibody (CD3-FITC / CD8-PE / CD4-PECy5) (Cytognos) is added to each sample tube. 3. 100 ƒY´l of blood sample is added to corresponding test tube by REVERSE PIPETTING and the tubes
107 的 PBMC 细胞用 100μl 的 Buffer 稀释(Buffer CI:含 0.5% BSA 的 PBS 缓冲液,1 mM EDTA) · 加入多种生物素化抗体,室温下孵育 5 分钟。生物素化的抗体包含以下几种,每种抗体 0.5 μg:CD235a (erythrocytes), CD14 (monocytes), CD3 (T cells), CD15 (granulocytes), CD19 (B cells), CD36 (thrombocytes), CD123
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