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Human IFNG ELISA kit

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  • ¥3900
  • RAYBIO
  • 15 pg/ml - 15000 pg/ml
  • ELH-IFNg-1
  • 2026年04月19日
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    上海研卉生物科技有限公司
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 样本：

      cell culture supernatants, plasma, serum, and lysate samples.

    • 标记物：

      Human IFNG ELISA kit

    • 适应物种：

      Human

    • 检测方法：

      ELISA

    • 检测范围：

      15 pg/ml - 15000 pg/ml

    • 库存：

      4周

    • 供应商：

      上海研卉生物科技有限公司

    • 规格：

      96T

    Human IFNG ELISA kit
    RayBio® Human IFN-gamma ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.
    文献：
    1. Sambataro M, Sambado L., Trevisiol E., et al. Proinsulin-expressing dendritic cells in type 2 neuropathic diabetic patients with and without foot lesions. FASEB J. 2018 Feb 12:fj201701279RR. doi: 10.1096/fj.201701279RR
      Species: Human
      Sample type: Plasma
    2. Plant, Ewan P., et al. "Influenza virus NS1 protein mutations at position 171 impact innate interferon responses by respiratory epithelial cells."?Virus research?240 (2017): 81-86.
      Species: Human
      Sample type: cell supernatants
    3. Sharm S., Shiman P., Rajendiran S., et al. Osteoarticular tuberculosis: in search of new biomarkers. European Orthopaedics and Traumatology. September 2015, Volume 6, Issue 3, pp 195-200
      Species: Human
      Sample type: Serum
    4. Shi L., Chen S., Zha X., et al. Enhancement of the TCR? Expression, Polyclonal Expansion, and Activation of T Cells from Patients with Acute Myeloid Leukemia After IL-2, IL-7, and IL-12 Induction. DNA Cell Biol. 2015 Jul;34(7):481-8. doi: 10.1089/dna.2015.2810.
      Species: Human
      Sample type: Conditioned Media
    5. Zaki M., Foad M., El-Deeb D., El-Kheir M. Diagnostic Performance of Gamma Interferon in Rapid Diagnosis of Tuberculosis Peritonitis. International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 4 Number 6 (2015) pp. 1-13
      Species: Human
      Sample type: Peritoneal Fluid
    6. Liu Y., Liao J., Zhao M., et al. Increased expression of TLR2 in CD4(+) T cells from SLE patients enhances immune reactivity and promotes IL-17 expression through histone modifications. Eur J Immunol. 2015 Sep;45(9):2683-93. doi: 10.1002/eji.201445219.
      Species: Human
      Sample type: Conditioned Media
    7. Yang Z., Lei Y., Chen C., et al. Roles of the programmed cell death 1, T cell immunoglobulin mucin-3, and cluster of differentiation 288 pathways in the low reactivity of invariant natural killer T cells after chronic hepatitis B virus infection. Archives of Virology October 2015, Volume 160, Issue 10, pp 2535-2545
      Species: Human
      Sample type: Conditioned Media
    8. Prandota J., Elleboudy N., Ismail K., et al. Increased Seroprevalence of Chronic Toxoplasmosis in Autistic Children: Special Reference to the Pathophysiology of IFN-? and NO Overproduction. Int. J. of Neurol. Res. 2015 September 1(3): 102-122. doi:10.17554/j.issn.2313-5611.2015.01.30
      Species: Human
      Sample type: Serum
    9. Moore Q., Smith C., Wolf S., et al. Examining the immune response of pneumolysin exposed human corneal epithelial cells in a keratitis model. BIOS 85(4):199-206. 2014. doi: http://dx.doi.org/10.1893/0005-3155-85.4.199
      Species: Human
      Sample type: Conditioned Media
    10. Abbas, H., Ali, K. et al. Comparison study between typhoid fever and tuberculosis patients to induceand produce of Th-1 cytokines (IL-18 and IFN-?). European Journal of Experimental Biology, 2014, 4(3):20-26
      Species: Human
      Sample type: Serum
      Target Species:
      • Human

      Assay Format

      Detection Range:
      15 pg/ml - 15000 pg/ml
      Recommended Dilution (serum & plasma):
      2 fold
      Sensitivity:
      15 pg/ml

      Need more sensitivity? Check out the new IQELISA™ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology.

      Specificity:
      This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
      Number of Targets Detected:
      1
      Species Detected:
      • Human
      Compatible Sample Types:
      • Cell Culture Supernatants
      • Cell Lysates
      • Plasma
      • Serum
      • Tissue Lysates
      Design Principle:
      • Sandwich-based
      Method of Detection:
      Colorimetric
      Quantitative/Semi-Quantitative:
      • Quantitative
      Solid Support:
      96-well Microplate

      Product Specifications

      Size:
      1, 2, or 5 x 96-Well Strip Microplate Kit

      Product Features

      Additional Notes
      • Strip plates and additional reagents allow for use in multiple experiments
      • Quantitative protein detection
      • Establishes normal range
      • The best products for confirmation of antibody array data

      Standard Curves

      产品细节图片1

      产品细节图片2

      Spiking & Recovery Results

      产品细节图片3

      Linearity Results

      产品细节图片4

      Application Notes

      Kit Components
      • Pre-Coated 96-well Strip Microplate
      • Wash Buffer
      • Stop Solution
      • Assay Diluent(s)
      • Lyophilized Standard
      • Biotinylated Detection Antibody
      • Streptavidin-Conjugated HRP
      • TMB One-Step Substrate
      Other Materials Required
      • Distilled or deionized water
      • Precision pipettes to deliver 2 µl to 1 µl volumes
      • Adjustable 1-25 µl pipettes for reagent preparation
      • 100 µl and 1 liter graduated cylinders
      • Tubes to prepare standard and sample dilutions
      • Absorbent paper
      • Microplate reader capable of measuring absorbance at 450nm
      • Log-log graph paper or computer and software for ELISA data analysis
      Protocol Outline
      1. Prepare all reagents, samples and standards as instructed in the manual.
      2. Add 100 µl of standard or sample to each well.
      3. Incubate 2.5 h at RT or O/N at 4°C.
      4. Add 100 µl of prepared biotin antibody to each well.
      5. Incubate 1 h at RT.
      6. Add 100 µl of prepared Streptavidin solution to each well.
      7. Incubate 45 min at RT.
      8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
      9. Incubate 30 min at RT.
      10. Add 50 µl of Stop Solution to each well.
      11. Read at 450 nm immediately.

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