ICC=1:50-200 IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分 子 量
215kDa
性 状
Lyophilized or Liquid
浓 度
1mg/ml
免 疫 原
KLH conjugated synthesised phosphopeptide derived from human APC1 around the phosphorylation site of Ser688
亚 型
IgG
纯化方法
affinity purified by Protein A
储 存 液
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
产品介绍
background: This gene encodes a subunit of the anaphase-promoting complex. This complex is an E3 ubiquitin ligase that regulates progression through the metaphase to anaphase portion of the cell cycle by ubiquitinating proteins which targets them for degradation. [provided by RefSeq, Dec 2011].
Function: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
Subunit: The APC/C is composed of at least 12 subunits.
Post-translational modifications: Phosphorylated. Phosphorylation on Ser-355 occurs specifically during mitosis.
Similarity: Belongs to the APC1 family. Contains 4 PC repeats.
Database links:
Entrez Gene: 64682 Human
Entrez Gene: 535565 Cow
Entrez Gene: 475750 Dog
Entrez Gene: 17222 Mouse
Omim: 608473 Human
SwissProt: E1BAB6 Cow
SwissProt: E2RLM2 Dog
SwissProt: Q9H1A4 Human
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.