- ¥2980
- LMAI Bio
- LM-4972R-FITC
- 中国/美国/欧洲
- 2025年07月14日
- ICC=1:50-200 IF=1:50-200
- Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep,
- Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep,
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海联迈生物工程有限公司
- 库存：
大量
- 靶点：
详见说明书
- 级别：
1
- 目录编号：
LM-4972R-FITC
- 克隆性：
多克隆
- 抗原来源：
Rabbit
- 保质期：
1年
- 抗体英文名：
Anti-PCK1/FITC
- 抗体名：
Anti-PCK1/FITC
- 标记物：
FITC标记
- 宿主：
Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep,
- 适应物种：
Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep,
- 免疫原：
详见说明书
- 亚型：
IGg
- 形态：
粉末、液体、冻干粉
- 应用范围：
ICC=1:50-200 IF=1:50-200
- 浓度：
1mg/ml
- 保存条件：
-20 °C
- 规格：
100ul
| 英文名称 | Anti-PCK1/FITC |
| 中文名称 | Anti-PCK1/FITC抗体 |
| 别 名 | PCK1; PEPC; GTP; MGC22652; PEP carboxykinase; PEPCK 1; PEPCK1; PEPCKC; Phosphoenolpyruvate carboxykinase 1; Phosphoenolpyruvate carboxylase; Phosphopyruvate carboxylase. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 肿瘤 细胞生物 免疫学 神经生物学 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep, |
| 产品应用 | ICC=1:50-200 IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 69kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PCK1 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: PCK1 is a main control point for the regulation of gluconeogenesis. This cytosolic enzyme, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of the corresponding gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. A mitochondrial isozyme has also been characterized. Function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. Subunit: Monomer. Subcellular Location: Cytoplasm. Tissue Specificity: Major sites of expression are liver, kidney and adipocytes. Post-translational modifications: Acetylation is increased on addition of glucose and appears to regulate the protein stability. DISEASE: Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (C-PEPCKD) [MIM:261680]. A metabolic disorder resulting from impaired gluconeogenesis. It is a rare disease with less than 10 cases reported in the literature. Clinical characteristics include hypotonia, hepatomegaly, failure to thrive, lactic acidosis and hypoglycemia. Autoposy reveals fatty infiltration of both the liver and kidneys. The disorder is transmitted as an autosomal recessive trait. Similarity: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family. Database links: Entrez Gene: 396458 Chicken Entrez Gene: 282855 Cow Entrez Gene: 403560 Dog Entrez Gene: 5105 Human Entrez Gene: 18534 Mouse Entrez Gene: 100144531 Pig Entrez Gene: 362282 Rat Omim: 614168 Human SwissProt: P05153 Chicken SwissProt: P35558 Human SwissProt: Q9Z2V4 Mouse SwissProt: P07379 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
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文献和实验对照。 二、注意事项 1、荧光染色后一般在 1h 内完成观察，或于 4℃ 保存 4h ，时间过长，可能会使荧光提前衰退。 2、每次试验均需设置以下三种对照： （1） 阳性对照：阳性血清+荧光标记物； （2）阴性对照：阴性血清+荧光标记物； （3）荧光标记物对照：PBS +荧光标记物。 三、免疫荧光双标的经验之谈 1、选取一抗时，要求来源于两种不同的动物，我用的是来源于家兔和大鼠的抗体，二抗则是不同荧光信号标记的，我用的是 donkey anti-rabbit-FITC（绿）和donkey anti
于细胞阳选，也能用于细胞阴选中。 为了评估 Strep-Tactin®TACS 琼脂糖柱细胞阴选的效率，实验从外周血单个核细胞（PBMC）样品中富集了 CD3- CD56+自然杀伤细胞（NK）。材料为Strep-Tactin®TACS 琼脂糖柱以及生物素化的抗体，仅 10 分钟，便完成了细胞阴选（图 1）。 流程如下： 阴选流程：生物素化抗体与 PBMC 孵育标记非目的细胞，加入至亲和层析柱后，即可收集目的细胞 实验方案 CD3- CD56+ NK 的细胞阴选 · 将 1 x
antibodies in 1% NGS/CMF-PBS. Make 4 mls anti-mouse FTIC diluted 1:150 Make 1.5 mls anti-rabbit FITC diluted 1:200 6. Bind secondary antibodies 45 minutes--use 300 ul/well. 7. Wash 3X with PBST, 5 minutes each wash-use ~1 ml/well. 8.
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