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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
江西江蓝纯生物试剂有限公司
- 库存:
大量
- 克隆性:
多克隆
- 保质期:
1年
- 抗体英文名:
Anti-HBG1/HBG2 antibody
- 宿主:
Rabbit
- 浓度:
0.6 mg/ml
- 保存条件:
-20°C
- 规格:
25 μl/100 μl/200 μl

Anti-HBG1/HBG2 antibody
Cat. No. JLC225246
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-HBG1/HBG2 rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Fusion protein of human HBG1/HBG2
Reactivity Human
Content 0.6 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol HBG1/HBG2
Full name hemoglobin subunit gamma 1/2
Synonyms fetal hemoglobin; HBGA; HBGR; HBG-T2; HSGGL1; PRO2979; TNCY; HBG-T1
Swissprot P69891/P69892
Target Background
The gamma globin genes (HBG1 and HBG2) are normally expressed in the fetal liver, spleen and bone marrow. Two gamma chains together with two alpha chains constitute fetal hemoglobin (HbF) which is normally replaced by adult hemoglobin (HbA) at birth. In some beta-thalassemias and related conditions, gamma chain production continues into adulthood. The two types of gamma chains differ at residue 136 where glycine is found in the G-gamma product (HBG2) and alanine is found in the A-gamma product (HBG1). The former is predominant at birth. The order of the genes in the beta-globin cluster is: 5'-epsilon -- gamma-G -- gamma-A -- delta -- beta--3'.
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human thyroid cancer
Recommended dilution: 40-200
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using ml225246(HBG1/HBG2 Antibody) at dilution 1/70, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:16 kDa
Positive control:Human plasma solution
Recommended dilution: 500-2000
Gel: 12%SDS-PAGE
Lysate: 40 μg
Lane: Human plasma solution
Primary antibody: ml225246(HBG1/HBG2 Antibody) at dilution 1/250
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 3 seconds
ELISA
Recommended dilution: 5000-10000
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