- 询价
- gelatins
- JLC224625
- 上海
- 2025年07月16日
- Rabbit
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
江西江蓝纯生物试剂有限公司
- 库存：
大量
- 克隆性：
多克隆
- 保质期：
1年
- 抗体英文名：
Anti-COPE antibody
- 宿主：
Rabbit
- 浓度：
0.2 mg/ml
- 保存条件：
-20°C
- 规格：
25 μl/100 μl/200 μl
Anti-COPE antibody
Cat. No. JLC224625
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-COPE rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Fusion protein of human COPE
Reactivity Human, Mouse
Content 0.2 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol COPE
Full name coatomer protein complex subunit epsilon
Synonyms epsilon-COP
Swissprot O14579
Target Background
The product of this gene is an epsilon subunit of coatomer protein complex. Coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles. It is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. Coatomer complex consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Alternatively spliced transcript variants encoding different isoforms have been identified.
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human liver cancer
Recommended dilution: 20-100
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using ml224625(COPE Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:34 kDa
Positive control:Hela，A375 and PC-3 cell lysates
Recommended dilution: 200-1000
Gel: 8%SDS-PAGE
Lysate: 40 μg
Lane 1-3: Hela，A375 and PC-3 cell lysates
Primary antibody: ml224625(COPE Antibody) at dilution 1/300
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 10 seconds
ELISA
Recommended dilution: 5000-10000
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