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鸡Mylk重组蛋白TelokinChicken Myosin

light chain kinase, smooth muscle(Mylk)蛋白
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  • ¥2328
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  • CN
  • CSB-EP320904CHb3
  • 2026年01月20日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.

    • 保质期

      Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

    • 英文名

      Recombinant Chicken Myosin light chain kinase, smooth muscle (Mylk), partial

    • 库存

      200

    • 供应商

      武汉华美生物工程有限公司

    • 规格

      20μg


    英文名称:

    Recombinant Chicken Myosin light chain kinase, smooth muscle (Mylk), partial

    品名简称:

    Recombinant Chicken Mylk protein, partial

    货号:

    CSB-EP320904CHb3

    规格:

    20μg

    价格:

    2328

    LC-MS/Western Blot:

    /

    SDS-PAGE:

    (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

    纯度:

    Greater than 90% as determined by SDS-PAGE.

    内毒素:

    Not test

    生物活性:

    /

    基因名:

    Mylk

    Uniprot No.:

    P11799

    别名:

    Telokin

    种属:

    Gallus gallus (Chicken)

    蛋白长度:

    Partial

    来源:

    E.coli

    分子量:

    52.9 kDa

    表达区域:

    1435-1722aa

    氨基酸序列:

    EVNYRTVTINTEQKVSDVYNIEERLGSGKFGQVFRLVEKKTGKVWAGKFFKAYSAKEKENIRDEISIMNCLHHPKLVQCVDAFEEKANIVMVLEMVSGGELFERIIDEDFELTERECIKYMRQISEGVEYIHKQGIVHLDLKPENIMCVNKTGTSIKLIDFGLARRLESAGSLKVLFGTPEFVAPEVINYEPIGYETDMWSIGVICYILVSGLSPFMGDNDNETLANVTSATWDFDDEAFDEISDDAKDFISNLLKKDMKSRLNCTQCLQHPWLQKDTKNMEAKKLSK

    蛋白标签:

    N-terminal 10xHis-SUMO-tagged and C-terminal MYC-tagged

    产品提供形式:

    Liquid or Lyophilized powder

    缓冲液:

    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

    储存条件:

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

    保质期:

    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

    货期:

    3-7 business days

    注意事项:

    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

    功能1:

    Phosphorylates a specific serine in the N-terminus of a myosin light chain, which leads to the formation of calmodulin/MLCK signal transduction complexes which allow selective transduction of calcium signals.

    功能2:

    Phosphorylates a specific serine in the N-terminus of a myosin light chain, which leads to the formation of calmodulin/MLCK signal transduction complexes which allow selective transduction of calcium signals.

    文献:

    "Multiple gene products are produced from a novel protein kinase transcription region." Watterson D.M., Collinge M., Lukas T.J., van Eldik L.J., Birukov K.G., Stepanova O.V., Shirinsky V.P. FEBS Lett. 373:217-220(1995)

    研究领域:

    Signal Transduction

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    相关实验
    • Purification of Myosin Light Chain Kinase

      RPM (4℃).Filter the supernatant through glass wool and discard the pellet (or save for light chain prep).Save 1 ml of supernatant (4℃)for assay.Add pepstatin and TLCK to final concentrations of 1 μg/ml and 40 Og/ml,respectively. Volume of supernatant

    • Isolation and Culture of Human Airway Smooth Muscle Cells

      doublings and express smooth muscle cell-specific markers such as myosin heavy chain, myosin light chain kinase, α-actin, caldesmon, and SM22α . In addition, the synthetic function of these cells appears to be comparable to those studied in vivo

    • Screening cDNA Libraries Using Partial Probes to Isolate Full-Length cDNAs from Vascular Cells

      within the library selected (1 ,2 ). As an example of both the utility and power of library screening, we will relate our own library screening efforts utilized to isolate the nonmuscle high molecular weight myosin light chain kinase isoform from a human umbilical

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    文献支持
    鸡Mylk重组蛋白TelokinChicken Myosin light chain kinase, smooth muscle(Mylk)蛋白
    ¥2328