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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Mycobacterium bovis Alpha-crystallin (hspX)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Mycobacterium bovis Alpha-crystallin (hspX)品名简称:
Recombinant Mycobacterium bovis hspX protein货号:
CSB-BP358651MVH规格:
20μg价格:
3168LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
hspXUniprot No.:
P0A5B8别名:
16 kDa antigen HSP 16.3种属:
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)蛋白长度:
Full Length of Mature Protein来源:
Baculovirus分子量:
18.1 kDa表达区域:
2-144aa氨基酸序列:
ATTLPVQRHPRSLFPEFSELFAAFPSFAGLRPTFDTRLMRLEDEMKEGRYEVRAELPGVDPDKDVDIMVRDGQLTIKAERTEQKDFDGRSEFAYGSFVRTVSLPVGADEDDIKATYDKGILTVSVAVSEGKPTEKHIQIRSTN蛋白标签:
C-terminal 9xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
/功能2:
/文献:
"Updated reference genome sequence and annotation of Mycobacterium bovis AF2122/97." Malone K.M., Farrell D., Stuber T.P., Schubert O.T., Aebersold R., Robbe-Austerman S., Gordon S.V. Genome Announc. 5:E00157-E00157(2017)研究领域:
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文献和实验[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
(GDI) alpha (ARHGDIA), mRNA 1358 *NM_000994 Homo sapiens ribosomal protein L32 (RPL32), mRNA 9523 *NM_022551 Homo sapiens ribosomal protein S18 (RPS18 ), mRNA 11261 *NM_007355 Homo sapiens heat shock 90kDa protein 1, beta (HSPCB ), mRNA 4119
剂如Triton X-100、脱氧胆酸盐和低浓度的变性剂如尿素充分洗涤去除杂质,这一步很重要,因为大肠杆菌外膜蛋白Omp T(37 KDa)在4-8mol/L尿素中具有蛋白水解酶活性,在包涵体的溶解和复性过程中可导致重组蛋白质的降解。 对于尿素和盐酸胍的选择: 尿素和盐酸胍属中强度变性剂,易经透析和超滤除去。它们对包涵体氢键有较强的可逆性变性作用,所需浓度尿素8-10M,盐酸胍6-8M。尿素溶解包涵体较盐酸胍慢而弱,溶解度为70-90%,尿素在作用时间较长或温度较高时会裂解形成氰酸盐,对重组蛋白质的氨
。胞浆内蛋白在蛋白酶作用下降解成肽段,依次被热休克蛋白HSP70和HSP90传递给膜上的TAP (tansporter associated with antigen processing) ,同时,在蛋白酶的作用下进一步被剪切成小肽。 小肽由TAP经HSP96传递给内质网内新合成的MHC I类分子上,最后由MHC I类分子将结合的抗原小肽托出细胞膜外。细胞外物质经专职APC吞噬(phagocytosis,指颗粒抗原)、胞饮(pinocytosis, 指可溶性抗原)或内吞(endocytosis
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