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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Rat BRAK protein (Cxcl14) (Active)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
500μg/100μg/5μg
| 规格: | 500μg | 产品价格: | ¥15708.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥6996.0 |
| 规格: | 5μg | 产品价格: | ¥852.0 |
纯度:
>95% as determined by SDS-PAGE.基因名:
Cxcl14Uniprot No.:
Q8K453别名:
/种属:
Rattus norvegicus (Rat)蛋白长度:
Full Length of Mature Protein来源:
E.Coli分子量:
9.4 kDa表达区域:
23-99aa氨基酸序列:
SKCKCSRKGPKIRYSDVKKLEMKPKYPHCEEKMVIITTKSMSRYRGQEHCLHPKLQSTKRFIKWYNAWNEKRRVYEE蛋白标签:
Tag-Free产品提供形式:
Lyophilized powder缓冲液:
Lyophilized from a 0.2 µm filtered PBS, pH 7.4货期:
5-10 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
/内毒素:
Less than 1.0 EU/µg as determined by LAL method.SDS-PAGE:
/LC-MS Image Description/Western Blot:
/Product types:
Active Protein & In Stock ProteinBiological_Activity:
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 1.0-10 ng/ml.Research Areas:
ImmunologyReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
/Function:
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文献和实验X-100或脱氧胆酸钠)和低浓度变性剂(2mol/L尿素或盐酸胍等)洗涤除去脂类和膜蛋白,这一步很重要,否则会导致包涵体溶解和复性的过程中重组蛋白质的降解[6、7、8]。 包涵体的溶解必须用很强的变性剂,如8mol/L尿素、6~8mol/L盐酸胍,通过离子间的相互作用破坏包涵体蛋白间的氢键而增溶蛋白。其中尿素的增溶效果稍差,异氰盐酸胍最强;去污剂,如SDS[7],可以破坏蛋白内的疏水键,可以增溶几乎所有的蛋白,但由于无法彻底去除而不允许用在制药行业中;酸,如70%甲酸[9],可以破坏蛋白
-2 and MMP-9可以应用明胶酶谱学方法,而MMP-3则不用明胶,而采用酪蛋白。如下:MMP enzyme expression was assayed by SDS-PAGE zymography using either gelatin or casein as MMP substrates. For gelatin-containing zymograms, equal volumes (10 ul) of samples normalized for protein concentration
and the cause of decreased refolding yields were included. Renaturation yield of recombinant protein have been improved by using some additives, such as molecular chaperone, small molecules. Key words inclusion body , protein , renaturation 外源基因在大肠杆菌中的
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