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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human C-C motif chemokine 25 protein (CCL25) (Active)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
5μg
英文名称:
Recombinant Human C-C motif chemokine 25 protein (CCL25) (Active)品名简称:
Recombinant Human CCL25 protein (Active)货号:
CSB-AP000981HU规格:
5μg价格:
852LC-MS/Western Blot:
/SDS-PAGE:
/纯度:
>97% as determined by SDS-PAGE.内毒素:
Less than 1.0 EU/μg as determined by LAL method.生物活性:
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 1.0-10 ng/ml.基因名:
CCL25Uniprot No.:
O15444别名:
Chemokine TECK, Thymus-expressed chemokine种属:
Homo sapiens (Human)蛋白长度:
Full Length of Mature Protein来源:
E.Coli分子量:
14.3 kDa表达区域:
24-150aa氨基酸序列:
M+QGVFEDCCLAYHYPIGWAVLRRAWTYRIQEVSGSCNLPAAIFYLPKRHRKVCGNPKSREVQRAMKLLDARNKVFAKLHHNTQTFQAGPHAVKKLSSGNSKLSSSKFSNPISSSKRNVSLLISANSGL蛋白标签:
Tag-Free产品提供形式:
Lyophilized powder缓冲液:
Lyophilized from a 0.2 µm filtered PBS, pH 7.4储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
5-10 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Isoform 2 is an antagonist of isoform 1. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.功能2:
Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Isoform 2 is an antagonist of isoform 1. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.文献:
/研究领域:
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文献和实验A chemokine-driven positive feedback loop organizes lymphoid follicles
of transfer, untreated spleen B cells expressed amounts of LT 1 2 equal to endogenous B cells (Fig. 3i ). By contrast, cells pretreated with pertussis toxin (PTX), an inhibitor of signalling by G i-coupled receptors including all known chemokine
reaction mixture to each tube in #3 and #4. Incubate 10 minutes at 25°C. Add 1 µl MMLV reverse transcriptase to the (+) RT tube and the positive and negative controls. Add 1 µl water to the (-) RT tube. Incubate 10 minutes at 25°C. Heat 40 minutes
Chemotaxis Assay Using Microchemotaxis Chambers (Modified Boyden Chamber)
containing pores of uniform size. A solution containing a chemokine or chemotactic factor is placed in the botton chamber and a cell suspension is placed in the upper chamber. Cells can migrate through the pores, across the thickness of the filter
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