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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Mouse Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial (Hadh)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Mouse Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial (Hadh)品名简称:
Recombinant Mouse Hadh protein货号:
CSB-EP713972MO规格:
20μg价格:
1836LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
HadhUniprot No.:
Q61425别名:
Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase;Short-chain 3-hydroxyacyl-CoA dehydrogenase种属:
Mus musculus (Mouse)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
49 kDa表达区域:
13-314aa氨基酸序列:
SSSSSASAAAKKILIKHVTVIGGGLMGAGIAQVAAATGHTVVLVDQTEDILAKSKKGIEESLKRMAKKKFTENPKAGDEFVEKTLSCLSTSTDAASVVHSTDLVVEAIVENLKLKNELFQRLDKFAAEHTIFASNTSSLQITNIANATTRQDRFAGLHFFNPVPMMKLVEVIKTPMTSQKTFESLVDFCKTLGKHPVSCKDTPGFIVNRLLVPYLIEAVRLHERGDASKEDIDTAMKLGAGYPMGPFELLDYVGLDTTKFILDGWHEMEPENPLFQPSPSMNNLVAQKKLGKKTGEGFYKYK蛋白标签:
N-terminal 6xHis-SUMO-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Plays an essential role in the mitochondrial beta-oxidation of short chain fatty acids. Exerts it highest activity toward 3-hydroxybutyryl-CoA.功能2:
Plays an essential role in the mitochondrial beta-oxidation of short chain fatty acids. Exerts it highest activity toward 3-hydroxybutyryl-CoA.文献:
Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013)研究领域:
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文献和实验[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
transporter-like protein (TETRAN), mRNA 935 NM_000182 Homo sapiens hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit (HADHA), mRNA 609 NM_003377 Homo sapiens vascular
所有的看家基因(housekeeping genes)的列表+引物设计服务
chain (MLC-B), mRNA 1231 NM_032348 Homo sapiens hypothetical protein MGC3047 (MGC3047), mRNA 553 NM_024798 Homo sapiens hypothetical protein FLJ13952 (FLJ13952), mRNA 2142 NM_021103 Homo
一篇很好的介绍RNAi实验原理与方法的文章,希望对您有所帮助
蛋白或者翻译起始复合物可能会影响siRNP核酸内切酶复合物结合mRNA从而影响siRNA的效果。 (2)将潜在的序列和相应的基因组数据库(人,或者小鼠,大鼠等等)进行比较,排除那些和其他编码序列/EST同源的序列。 例如使用BLAST( www.ncbi.nlm.nih.gov/BLAST/) (3)选出合适的目标序列进行合成。通常一个基因需要设计多个靶序列的siRNA,以找到最有效的siRNA序列。 3.阴性对照 一个完整的siRNA实验应该有阴性对照,作为阴性对照的siRNA应该和选中
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