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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海联迈生物工程有限公司
- 库存:
大量
- 靶点:
详见说明书
- 级别:
1
- 目录编号:
LM-0245R-FITC
- 克隆性:
多克隆
- 抗原来源:
Rabbit
- 保质期:
1年
- 抗体英文名:
Anti-ELP1/FITC
- 抗体名:
Anti-ELP1/FITC
- 标记物:
FITC标记
- 宿主:
Arabidopsis Thaliana,
- 适应物种:
Arabidopsis Thaliana,
- 免疫原:
详见说明书
- 亚型:
IGg
- 形态:
粉末、液体、冻干粉
- 应用范围:
IF=1:50-200
- 浓度:
1mg/ml
- 保存条件:
-20 °C
- 规格:
100ul
| 英文名称 | Anti-ELP1/FITC |
| 中文名称 | FITC标记的拟南芥IKI3抗体 |
| 别 名 | IKI3 family protein; Elongator complex protein 1; AtELP1; Elongator component 1; Protein ABA-OVERLY SENSITIVE 1; Protein ELONGATA 2; ELP1_ARATH. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 产品类型 | 植物抗体 |
| 研究领域 | 植物 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Arabidopsis Thaliana, |
| 产品应用 | IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 147kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from Arabidopsis thaliana IKI3 family protein C-terminus |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: IKI3 family is the Members of this family are components of the elongator multi-subunit component of a novel RNA polymerase II holoenzyme for transcriptional elongation. Function: Acts as subunit of the RNA polymerase II elongator complex, which is a histone acetyltransferase component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Elongator may play a role in chromatin remodeling and is involved in acetylation of histones H3 and probably H4 (By similarity). Promotes organs development by modulating cell division rate. Required for auxin distribution or signaling. Prevents abscisic acid (ABA) signaling leading to stomatal closure and seedling growth inhibition. Involved in oxidative stress signaling. Prevents anthocyanins accumulation. Subunit: Component of the RNA polymerase II elongator complex (Elongator) made of at least six subunits, ELP1/ELO2, ELP2, ELP3/ELO3, ELP4/ELO1, ELP5, and ELP6. Subcellular Location: Cytoplasm (By similarity). Nucleus (By similarity). Tissue Specificity: Expressed in meristematic tissues of in roots, stems, leaves, seedlings, cotyledons, guard cells, floral buds, flowers, siliques, and shoot apices. Similarity: Belongs to the ELP1/IKA1 family. Contains 7 WD repeats. Database links: UniProtKB/Swiss-Prot: Q9FNA4.1 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 拟南芥是一种十字花科植物,广泛用于植物遗传学、发育生物学和分子生物学的研究,已成为一种典型的模式植物。 由于这种植物的全部基因组测序已经完成,因此可以预测,拟南芥在植物学所有领域的研究中将发挥更大的作用。虽然这种植物在许多方面"简单",但它的大多数基因与其他"复杂"的植物基因具有很高的同源性。 该植物具有以下特点: (1)形态个体小,高度只有3Ocm左右; (2)生长周期快,从播种到收获种子一般只需6周左右; (3)种子多,每株每代可产生数千粒种子; (4)形态特征简单; (5)基因组小,只有5对染色体。 |
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文献和实验当FITC在碱性溶液中与抗体蛋白反应时,主要是蛋白质上赖氨酸的r氨基与荧光素的硫碳胺键(thiocarbmide)结合,形成FITC-蛋白质结合物,即荧光抗体或荧光结合物。一个IgG分子中有86个赖氨酸残基,一般最多能结合15~20个,一个IgG分子可结合2~8个分子的FITC,其反应式如下FITC-N=C=S + N-H2-蛋白质 → FITC-NS-C-N-H2-蛋白质常用Marsshall(1958)法标记荧光抗体,也可以根据条件采用Chadwick等标记法或Clark
ml三蒸水中即成; 方法与步骤: 根据Marshall氏法高效价的抗人球蛋白兔免疫血清,分离球蛋白。 1. 用0.15 mol/L NaCl的盐水及0.15 mol/L pH9.0的NaHCO3-Na2CO3缓冲液稀释使每毫升内含抗体10mg,缓冲液为总量的10%; 2. 将以上溶液降温至4℃,按蛋白:荧光素=50—80mg:1mg的比例加入异硫氰酸荧光素,在0—4℃下电磁搅拌12—14h; 3.用半饱和硫酸铵将标记球蛋白
5. 过柱。取透析过夜的标记物,过葡萄糖凝胶G-25或G-50柱,分离出游离荧光素,收集标记的荧光抗体进行鉴定。
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