Product overview Description Anti-NOX5 rabbit polyclonal antibody Applications ELISA, WB Immunogen Fusion protein of human NOX5 Reactivity Human Content 0.1 mg/ml Host species Rabbit Ig class Immunogen-specific rabbit IgG Purification Antigen affinity purification
Target information Symbol NOX5 Full name NADPH oxidase, EF-hand calcium binding domain 5 Synonyms Swissprot Q96PH1
Target Background This gene is predominantly expressed in the testis and lymphocyte-rich areas of spleen and lymph nodes. It encodes a calcium-dependen NADPH oxidase that generates superoxide, and functions as a calcium-dependent proton channel that may regulate redox-dependent processes in lymphocytes and spermatozoa. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Applications Western blotting Predicted band size:86 kDa Positive control:293T and hela cells, human testis tissue, skov3, Raji and Jurkat cells Recommended dilution: 500-2000
Gel: 6%SDS-PAGE Lysate: 40 μg Lane 1-6: 293T cells, hela cells, human testis tissue, skov3 cells, Raji cells, Jurkat cells Primary antibody: ml222775(NOX5 Antibody) at dilution 1/200 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 20 seconds