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- 文献和实验
- 技术资料
- 免疫原:
Recombinant Human Purine-rich element-binding protein gamma protein (242-345AA)
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Upon receipt, store at -20℃ or -80℃. Avoid repeated freeze.
- 克隆性:
Polyclonal
- 标记物:
HRP
- 适应物种:
Human
- 保质期:
6个月
- 抗原来源:
Homo sapiens (Human)
- 目录编号:
Q9UJV8
- 级别:
优
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 宿主:
Rabbit
- 应用范围:
ELISA
- 浓度:
>95%,Protein G purified
- 靶点:
PURG
- 抗体英文名:
PURG Antibody, HRP conjugated
- 抗体名:
PURG antibody;Purine-rich element-binding protein gamma antibody;Purine-rich element-binding protein G antibody
- 规格:
100μg/50μg
| 规格: | 100μg | 产品价格: | ¥1320.0 |
|---|---|---|---|
| 规格: | 50μg | 产品价格: | ¥880.0 |
保存缓冲液
Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
功能
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文献和实验ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
2c + ++ Sources of information: Akerstrom, B., Brodin, T., Reis, K., and Bjorck, L. 1985. Protein G: A powerful tool for binding and detection of monoclonal and polyclonal antibodies. J. Immunol. 135:2589-2592. Akerstrom, B. and Bjorck, L. 1986
Antibody Purification: Affinity Chromatography Protein A and Protein G Sepharose
Affinity chromatography relies on the reversible interaction between a protein and a specific ligand immobilized in a chromatographic matrix. The sample is applied under conditions that favor specific binding to the ligand as the result
Coupling Antibodies to Protein A or G
1. use 2 mg of antibody per ml wet beads (use appropriate antibody/protein A or G combination). 2. mix antibodies with beads and bind at room temperature for at least 1 hr (on roller). 3. wash the beads twice with 10 volumes borate buffer, spin
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