- 询价
- gelatins
- JLC222587
- 上海
- 2025年07月11日
- Rabbit
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
江西江蓝纯生物试剂有限公司
- 库存：
大量
- 克隆性：
多克隆
- 保质期：
1年
- 抗体英文名：
Anti-KIF20A antibody
- 宿主：
Rabbit
- 浓度：
0.2 mg/ml
- 保存条件：
-20°C
- 规格：
25 μl/100 μl/200 μl
Cat. No. JLC222587
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-KIF20A rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Fusion protein of human KIF20A
Reactivity Human, Mouse
Content 0.2 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol KIF20A
Full name kinesin family member 20A
Synonyms MKLP2; RAB6KIFL
Swissprot O95235
Target Background
KIF20A (kinesin family member 20A), also known as Rabkinesin-6, RAB6KIFL (Rab6-interacting kinesin-like protein), GG10_2 or MKLP2 (mitotic kinesin-like protein 2), is a 890 amino acid protein that contains one kinesin-motor domain and belongs to the Kinesin-like protein family. KIF20A locates to the Golgi apparatus and interacts with guanosine triphosphate (GTP)-bound forms of RAB 6. KIF20A may be responsible for the retrograde RAB 6 regulated transport of Golgi membranes and related vesicles along microtubules.
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm or Nucleus
Positive control: Human breast cancer
Recommended dilution: 50-200 Predicted cell location: Cytoplasm or Nucleus
Positive control: Human thyroid cancer
Recommended dilution: 50-200
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using ml222587(KIF20A Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using ml222587(KIF20A Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:100 kDa
Positive control:K562, 293T and hela cells
Recommended dilution: 200-1000
Gel: 6%SDS-PAGE
Lysate: 40 μg
Lane 1-3: K562 cells, 293T cells, hela cells
Primary antibody: ml222587(KIF20A Antibody) at dilution 1/400
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 10 seconds
ELISA
Recommended dilution: 1000-2000
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