Product overview Description Anti-IL27RA rabbit polyclonal antibody Applications ELISA, WB, IHC Immunogen Fusion protein of human IL27RA Reactivity Human Content 0.3 mg/ml Host species Rabbit Ig class Immunogen-specific rabbit IgG Purification Antigen affinity purification
Target information Symbol IL27RA Full name interleukin 27 receptor, alpha Synonyms CRL1; TCCR; WSX1; IL27R; IL-27RA; zcytor1 Swissprot Q6UWB1
Target Background In mice, CD4+ helper T-cells differentiate into type 1 (Th1) cells, which are critical for cell-mediated immunity, predominantly under the influence of IL12. Also, IL4 influences their differentiation into type 2 (Th2) cells, which are critical for most antibody responses. Mice deficient in these cytokines, their receptors, or associated transcription factors have impaired, but are not absent of, Th1 or Th2 immune responses. This gene encodes a protein which is similar to the mouse T-cell cytokine receptor Tccr at the amino acid level, and is predicted to be a glycosylated transmembrane protein.
Applications Immunohistochemistry Predicted cell location: Cytoplasm Positive control: Human liver cancer Recommended dilution: 50-200 Predicted cell location: Cytoplasm Positive control: Human brain Recommended dilution: 50-200
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using ml222541(IL27RA Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using ml222541(IL27RA Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200) Western blotting Predicted band size:69 kDa Positive control:Jurkat cells Recommended dilution: 200-1000
Gel: 8%SDS-PAGE Lysate: 50 μg Lane: Jurkat cells Primary antibody: ml222541(IL27RA Antibody) at dilution 1/200 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 20 seconds